Geoffrey van Dover, Josh Javor, Jourdan K Ewoldt, Mikhail Zhernenkov, Patryk Wąsik, Guillaume Freychet, Josh Lee, Dana Brown, Christopher S Chen, David J Bishop
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引用次数: 0
摘要
了解人类诱导多能干细胞衍生心肌细胞的结构和功能发育情况，对工程化心脏组织进行药物测试、疾病建模和设计疗法至关重要。在这里，我们使用一种不常用于生物材料的方法--小角 X 射线散射--来表征三维工程组织中人类诱导多能干细胞衍生心肌细胞成熟初期的结构发展。X 射线散射实验方法能够可靠地表征心肌细胞肌丝间距随成熟时间的变化。在十天的时间里，随着组织从播种后的初始状态逐渐成熟，肌丝晶格间距单调地减小。组织中网格位置的间距可视化提供了一种表征心肌细胞肌丝成熟和组织的方法，并有可能帮助阐明病理生理学和疾病进展的机制，从而激发干细胞工程中新的生物学假设。
Structural maturation of myofilaments in engineered 3D cardiac microtissues characterized using small angle x-ray scattering.
Understanding the structural and functional development of human-induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs) is essential to engineering cardiac tissue that enables pharmaceutical testing, modeling diseases, and designing therapies. Here we use a method not commonly applied to biological materials, small angle x-ray scattering, to characterize the structural development of hiPSC-CMs within three-dimensional engineered tissues during their preliminary stages of maturation. An x-ray scattering experimental method enables the reliable characterization of the cardiomyocyte myofilament spacing with maturation time. The myofilament lattice spacing monotonically decreases as the tissue matures from its initial post-seeding state over the span of 10 days. Visualization of the spacing at a grid of positions in the tissue provides an approach to characterizing the maturation and organization of cardiomyocyte myofilaments and has the potential to help elucidate mechanisms of pathophysiology, and disease progression, thereby stimulating new biological hypotheses in stem cell engineering.
期刊介绍：
Physical Biology publishes articles in the broad interdisciplinary field bridging biology with the physical sciences and engineering. This journal focuses on research in which quantitative approaches – experimental, theoretical and modeling – lead to new insights into biological systems at all scales of space and time, and all levels of organizational complexity.
Physical Biology accepts contributions from a wide range of biological sub-fields, including topics such as:
molecular biophysics, including single molecule studies, protein-protein and protein-DNA interactions
subcellular structures, organelle dynamics, membranes, protein assemblies, chromosome structure
intracellular processes, e.g. cytoskeleton dynamics, cellular transport, cell division
systems biology, e.g. signaling, gene regulation and metabolic networks
cells and their microenvironment, e.g. cell mechanics and motility, chemotaxis, extracellular matrix, biofilms
cell-material interactions, e.g. biointerfaces, electrical stimulation and sensing, endocytosis
cell-cell interactions, cell aggregates, organoids, tissues and organs
developmental dynamics, including pattern formation and morphogenesis
physical and evolutionary aspects of disease, e.g. cancer progression, amyloid formation
neuronal systems, including information processing by networks, memory and learning
population dynamics, ecology, and evolution
collective action and emergence of collective phenomena.
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