利用果蝇触角和大脑样本进行染色质免疫沉淀（ChIP）

Cold Spring Harbor protocols Pub Date : 2025-01-02 DOI:10.1101/pdb.prot108142

Chengcheng Du, Pelin Volkan

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引用次数: 0

摘要

染色质免疫沉淀（ChIP）是研究 DNA 序列上蛋白质结合模式或基因组中具有不同标记的组蛋白分布的常用方法。本方案详细概述了从果蝇神经系统（特别是触角和大脑）样本中提取已固定和剪切的 ChIP 程序中的免疫沉淀部分。在对样本大小和其他相关参数进行优化后，这些方法可应用于其他感兴趣的组织。

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Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples.

Chromatin immunoprecipitation (ChIP) is a common approach for studying the binding pattern of proteins on DNA sequences or the landscape of histones with different marks throughout the genome. ChIP is used on various organisms, including Drosophila This protocol provides a detailed overview of the immunoprecipitation portion of a ChIP procedure from samples of Drosophila nervous systems, specifically antennae and brains, that have already been fixed and sheared. These methods can be applied to other tissues of interest after optimizing for sample size and other relevant parameters.

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来源期刊

Cold Spring Harbor protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

3.00

自引率

0.00%

发文量

163

期刊介绍： Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.