量化小胶质细胞形态:洞察功能

IF 3.4 3区 医学 Q3 IMMUNOLOGY
Tabitha R F Green, Rachel K Rowe
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引用次数: 0

摘要

小胶质细胞是中枢神经系统(CNS)特有的特化免疫细胞。小胶质细胞的形态具有高度可塑性,会因损伤或感染而迅速变化。对不断变化的小胶质细胞形态进行定性和定量测量被认为是许多以小胶质细胞为中心的研究的基石。小胶质细胞独特的形态变化是炎症和组织损伤严重程度的有效标志。虽然已经记录了一系列与损伤相关的小胶质细胞形态,但这些不同形态的确切功能还不完全清楚。在本综述中,我们将讨论小胶质细胞形态并不等同于小胶质细胞功能,但形态学结果可用于推断小胶质细胞的功能。要全面检查小胶质细胞的反应状态,应将组织学和遗传学方法结合起来。不过,高质量的免疫组化分析也不容忽视,因为它们可以简明扼要地回答许多研究问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantifying microglial morphology: an insight into function.

Microglia are specialized immune cells unique to the central nervous system (CNS). Microglia have a highly plastic morphology that changes rapidly in response to injury or infection. Qualitative and quantitative measurements of ever-changing microglial morphology are considered a cornerstone of many microglia-centric research studies. The distinctive morphological variations seen in microglia are a useful marker of inflammation and severity of tissue damage. Although a wide array of damage-associated microglial morphologies has been documented, the exact functions of these distinct morphologies are not fully understood. In this review, we discuss how microglia morphology is not synonymous with microglia function, however, morphological outcomes can be used to make inferences about microglial function. For a comprehensive examination of the reactive status of a microglial cell, both histological and genetic approaches should be combined. However, the importance of quality immunohistochemistry-based analyses should not be overlooked as they can succinctly answer many research questions.

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来源期刊
CiteScore
8.40
自引率
2.20%
发文量
101
审稿时长
3-8 weeks
期刊介绍: Clinical & Experimental Immunology (established in 1966) is an authoritative international journal publishing high-quality research studies in translational and clinical immunology that have the potential to transform our understanding of the immunopathology of human disease and/or change clinical practice. The journal is focused on translational and clinical immunology and is among the foremost journals in this field, attracting high-quality papers from across the world. Translation is viewed as a process of applying ideas, insights and discoveries generated through scientific studies to the treatment, prevention or diagnosis of human disease. Clinical immunology has evolved as a field to encompass the application of state-of-the-art technologies such as next-generation sequencing, metagenomics and high-dimensional phenotyping to understand mechanisms that govern the outcomes of clinical trials.
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