Xiaoyu Ge, Junlin Chen, Ouqi Li, Min Zou, Baolong Tao, Lun Zhao, Jing Wen, Bin Yi, Jinxing Tu, Jinxiong Shen
{"title":"ORF138 会导致舌苔脂质代谢异常,从而导致甘蓝型油菜的奥谷细胞质雄性不育症","authors":"Xiaoyu Ge, Junlin Chen, Ouqi Li, Min Zou, Baolong Tao, Lun Zhao, Jing Wen, Bin Yi, Jinxing Tu, Jinxiong Shen","doi":"10.1016/j.jia.2024.03.009","DOIUrl":null,"url":null,"abstract":"Plant cytoplasmic male sterility is caused by mutations and rearrangements of mitochondrial genes. It serves as a significant way to utilize hybrid vigor to enhance crop yield. Ogu CMS is a natural cytoplasmic male sterility type discovered in radishes, being successfully transferred to rapeseed and cruciferous vegetables. However, current studies lack depth in exploring the molecular mechanisms of its male sterility. In this study, we confirmed is the causal gene for Ogu CMS through genetic transformation in . Transcriptome analysis of aborted anthers in different stages suggested differentially expressed genes (DEGs) are mainly enriched in pathways such as glycerophospholipid metabolism and arginine and proline metabolism. It reveals that key genes involved in lipid metabolism pathways are significantly down-regulated in the sterile line (OguA), including , localized within the tapetum mitochondrial and endoplasmic reticulum. This could lead to changes in the metabolism of substances like acylglycerols within the tapetum, causing disruptions in lipid metabolism. This is consistent with morphological and subcellular structural changes in the tapetum and microspore cells as observed in the transmission electron microscopy. This abnormal lipid metabolism may trigger specific ROS accumulation in an oxidative stress response, ultimately leading to aborted microspore. Our study based on transcriptome has deepened our understanding of the molecular mechanisms in Ogu CMS.","PeriodicalId":16305,"journal":{"name":"Journal of Integrative Agriculture","volume":"37 1","pages":""},"PeriodicalIF":4.6000,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ORF138 causes abnormal lipid metabolism in the tapetum leading to Ogu cytoplasmic male sterility in Brassica napus\",\"authors\":\"Xiaoyu Ge, Junlin Chen, Ouqi Li, Min Zou, Baolong Tao, Lun Zhao, Jing Wen, Bin Yi, Jinxing Tu, Jinxiong Shen\",\"doi\":\"10.1016/j.jia.2024.03.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Plant cytoplasmic male sterility is caused by mutations and rearrangements of mitochondrial genes. It serves as a significant way to utilize hybrid vigor to enhance crop yield. Ogu CMS is a natural cytoplasmic male sterility type discovered in radishes, being successfully transferred to rapeseed and cruciferous vegetables. However, current studies lack depth in exploring the molecular mechanisms of its male sterility. In this study, we confirmed is the causal gene for Ogu CMS through genetic transformation in . Transcriptome analysis of aborted anthers in different stages suggested differentially expressed genes (DEGs) are mainly enriched in pathways such as glycerophospholipid metabolism and arginine and proline metabolism. It reveals that key genes involved in lipid metabolism pathways are significantly down-regulated in the sterile line (OguA), including , localized within the tapetum mitochondrial and endoplasmic reticulum. This could lead to changes in the metabolism of substances like acylglycerols within the tapetum, causing disruptions in lipid metabolism. This is consistent with morphological and subcellular structural changes in the tapetum and microspore cells as observed in the transmission electron microscopy. This abnormal lipid metabolism may trigger specific ROS accumulation in an oxidative stress response, ultimately leading to aborted microspore. Our study based on transcriptome has deepened our understanding of the molecular mechanisms in Ogu CMS.\",\"PeriodicalId\":16305,\"journal\":{\"name\":\"Journal of Integrative Agriculture\",\"volume\":\"37 1\",\"pages\":\"\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-03-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Integrative Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1016/j.jia.2024.03.009\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Agriculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.jia.2024.03.009","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
ORF138 causes abnormal lipid metabolism in the tapetum leading to Ogu cytoplasmic male sterility in Brassica napus
Plant cytoplasmic male sterility is caused by mutations and rearrangements of mitochondrial genes. It serves as a significant way to utilize hybrid vigor to enhance crop yield. Ogu CMS is a natural cytoplasmic male sterility type discovered in radishes, being successfully transferred to rapeseed and cruciferous vegetables. However, current studies lack depth in exploring the molecular mechanisms of its male sterility. In this study, we confirmed is the causal gene for Ogu CMS through genetic transformation in . Transcriptome analysis of aborted anthers in different stages suggested differentially expressed genes (DEGs) are mainly enriched in pathways such as glycerophospholipid metabolism and arginine and proline metabolism. It reveals that key genes involved in lipid metabolism pathways are significantly down-regulated in the sterile line (OguA), including , localized within the tapetum mitochondrial and endoplasmic reticulum. This could lead to changes in the metabolism of substances like acylglycerols within the tapetum, causing disruptions in lipid metabolism. This is consistent with morphological and subcellular structural changes in the tapetum and microspore cells as observed in the transmission electron microscopy. This abnormal lipid metabolism may trigger specific ROS accumulation in an oxidative stress response, ultimately leading to aborted microspore. Our study based on transcriptome has deepened our understanding of the molecular mechanisms in Ogu CMS.
期刊介绍:
Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.