优化棉花中苏云金芽孢杆菌(Bt)蛋白的浓度:协调调节外源氨基酸和乙二胺四乙酸,减少棉铃和叶片毒素的时空变异性

IF 4.6 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Zhenyu Liu, Shu Dong, Yuting Liu, Hanjia Li, Fuqin Zhou, Junfeng Ding, Zixu Zhao, Yinglong Chen, Xiang Zhang, Yuan Chen, Dehua Chen
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引用次数: 0

摘要

在棉铃形成阶段,棉铃的杀虫蛋白表达量最低。不同器官对昆虫的抗性差异显著,这给防治棉铃虫带来了挑战。因此,我们在 2020-2021 年棉花生长季节设计了一种策略,以协调蛋白质合成的增强和降解的减弱。两种 Bt 栽培品种(即杂交 Sikang 3 和常规 Sikang 1)被用作试验材料。试验采用了三种处理方法:CK(对照)、T1(氨基酸)和 T2(氨基酸和乙二胺四乙酸)。这些处理均在盛花期进行。结果表明,与 CK 组相比,在 T1 和 T2 处理中,棉铃及其副叶的 Bt 蛋白质含量均显著增加。棉铃和棉叶的 Bt 蛋白含量分别增加了 67.5%和 21.7%。此外,棉铃及其副叶的 Bt 蛋白质含量差距明显缩小了 31.2%。相关分析表明,提高 Bt 蛋白含量的主要生理机制包括蛋白质合成增加和蛋白质分解减少,与 Bt 基因表达水平无关。逐步回归和路径分析显示,提高可溶性蛋白质含量和转氨酶活性,同时降低分解酶活性,有助于提高 Bt 蛋白质含量。因此,氨基酸和乙二胺四乙酸的协调调节是一种策略,能够提高 Bt 棉花的整体抗性,缓解棉铃和棉叶中 Bt 毒素浓度的时空变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimizing Bacillus thuringiensis (Bt) protein concentration in cotton: Coordinated regulation of exogenous amino acids and EDTA to reduce spatiotemporal variability in boll and leaf toxin
During the boll formation stage, cotton bolls exhibit the lowest expression of insecticidal proteins. Insect resistance varies notably among different organs, posing challenges for controlling cotton bollworms. Consequently, a strategy was designed in the 2020-2021 cotton growing season to coordinate the enhancement of protein synthesis and the attenuation of degradation. Two Bt cultivars of . , namely the hybrid Sikang 3 and the conventional Sikang 1, were used as test materials. Three treatments were implemented: CK (the control), T1 (amino acids), and T2 (amino acids and EDTA). These treatments were applied at the peak flowering period. The results show that, in comparison to the CK group, Bt protein content significantly increased both cotton bolls and their subtending leaves under T1 and T2 treatments. The maximum increase observed was 67.5% in cotton bolls and 21.7% in leaves. Moreover, the disparity in Bt protein content between cotton bolls and their subtending leaves notably decreased by 31.2%. Correlation analysis suggests that the primary physiological mechanisms for augmenting Bt protein content involve increased protein synthesis and decreased protein catabolism, independent of Bt gene expression levels. Stepwise regression and path analysis reveal that elevating soluble protein content and transaminase activity, while reducing catabolic enzyme activity, are instrumental in enhancing Bt protein content. Consequently, the coordinated regulation of amino acids and EDTA emerges as a strategy capable of improving the overall resistance of Bt cotton and mitigating spatiotemporal variations in Bt toxin concentrations in both cotton bolls and leaves.
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来源期刊
Journal of Integrative Agriculture
Journal of Integrative Agriculture AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
7.90
自引率
4.20%
发文量
4817
审稿时长
3-6 weeks
期刊介绍: Journal of Integrative Agriculture publishes manuscripts in the categories of Commentary, Review, Research Article, Letter and Short Communication, focusing on the core subjects: Crop Genetics & Breeding, Germplasm Resources, Physiology, Biochemistry, Cultivation, Tillage, Plant Protection, Animal Science, Veterinary Science, Soil and Fertilization, Irrigation, Plant Nutrition, Agro-Environment & Ecology, Bio-material and Bio-energy, Food Science, Agricultural Economics and Management, Agricultural Information Science.
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