挖掘外来种质，遗传改良大豆（Glycine max）蛋白质的数量和质量

IF 1.5 4区农林科学 Q2 AGRONOMY

Plant Breeding Pub Date : 2024-03-06 DOI:10.1111/pbr.13165

Renan Souza, Blair Buckley, M. A. Rouf Mian, Zenglu Li

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引用次数: 0

摘要

豆粕是动物饲料的主要蛋白质来源，但其必需氨基酸半胱氨酸（Cys）和蛋氨酸（Met）的含量较低。在这项研究中，将外来种质（PI 399000）与'Woodruff'杂交，培育出 F5 重组近交系（RIL）群体，用于绘制种子成分的数量性状位点图。该群体在六种环境中生长，用近红外光谱测定蛋白质、油脂、Cys 和 Met。用 SoySNP6K BeadChip 对 RIL 进行基因分型，分析了 1865 个 SNPs。QTL 分析在至少 5 个环境中发现了染色体（Chrs）6、15 和 17 上的 3 个蛋白质 QTL 位点；在所有环境中发现了染色体 14 和 17 上的 2 个油分 QTL 位点；在至少 3 个环境中发现了染色体 3、6 和 10 上的 3 个 Cys 和 Met QTL 位点；在所有环境中发现了染色体 17 和 20 上的 2 个种子大小 QTL 位点。蛋白质和 Cys + Met QTLs 的叠加可同时增加这两个性状，并鉴定出 13 个种子成分得到改善的育种品系。与 QTLs 相连的标记可用于帮助培育具有更好膳食质量的栽培品种。

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Mining exotic germplasm for genetic improvement of protein quantity and quality in soybean (Glycine max)

Soybean meal is the main protein source for animal feed, but it has low content of the essential amino acids cysteine (Cys) and methionine (Met). In this research, an exotic germplasm (PI 399000) was crossed with ‘Woodruff’ to develop an F5‐derived recombinant inbred line (RIL) population for mapping quantitative trait loci for seed composition. The population was grown in six environments, and protein, oil, Cys, and Met were determined with near‐infrared spectroscopy. RILs were genotyped with the SoySNP6K BeadChip, and 1865 SNPs were used for analysis. QTL analysis identified three loci on chromosomes (Chrs) 6, 15 and 17 in at least five environments for protein; two QTLs on Chrs 14 and 17 in all environments for oil; three QTLs on Chrs 3, 6 and 10 for Cys and Met in at least three environments; and two QTLs for seed size on Chrs 17 and 20 in all environments. Stacking of protein and Cys + Met QTLs can increase both traits simultaneously, and 13 breeding lines were identified with improved seed composition. The markers linked to the QTLs can be used to assist the development of cultivars with improved meal quality.

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来源期刊

Plant Breeding 农林科学-农艺学

CiteScore

4.40

自引率

5.00%

发文量

审稿时长

3.0 months

期刊介绍： PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.