M. A. Parsadanyan, M. A. Shahinyan, S. V. Grigoryan, M. S. Mikaelyan, G. H. Poghosyan, V. P. Kalantaryan, P. O. Vardevanyan
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引用次数: 0
摘要
摘要 在频率为 41.8 和 51.8 GHz 的毫米波电磁波（MM EMW）的影响下，通过紫外变性和光谱方法（吸收、荧光和圆二色性（CD））研究了营养防腐剂和染料赤藓红 B 与牛血清白蛋白（BSA）的相互作用。我们的研究结果表明，用频率为 51.8 GHz 的 MM EMW 对 BSA-erythrosine B 复合物进行辐照后，其变性参数在实验误差范围内与未辐照样品的变性参数没有差异。我们还发现，在 51.8 GHz 频率辐照的影响下，BSA 的构象没有发生变化。此外，在 MM EMW 的影响下，BSA 和赤藓红 B 之间产生了额外的氢键和范德华相互作用，因此复合体的形成过程在热力学上是有益的。
Interaction of Serum Albumin with Erythrosine B under the Influence of Millimeter-Range Electromagnetic Waves
The interaction of nutritional preservative and dye erythrosine B with bovine serum albumin (BSA) under the influence of millimeter range electromagnetic waves (MM EMW) with frequencies 41.8 and 51.8 GHz has been studied by the UV-denaturation and spectroscopic methods (absorption, fluorescence, and circular dichroism (CD)). We show that the irradiation by MM EMW with the frequency 51.8 GHz stabilizes the complex BSA-erythrosine B. At the same time, denaturation parameters of the complex at the irradiation of BSA solution with the frequency 41.8 GHz do not differ from those obtained for non-irradiated samples within the limits of experimental error. We also show that under the influence of the irradiation by 51.8 GHz frequency there is no conformational change of BSA. However, a change in the protein conformation takes place in the case of the complex BSA-erythrosine B. In addition, the complex-formation process is thermodynamically beneficial and implemented because of the additional hydrogen bonds and van-der-Waals interaction between BSA and erythrosine B under the influence of MM EMW.
期刊介绍：
Journal of Contemporary Physics (Armenian Academy of Sciences) is a journal that covers all fields of modern physics. It publishes significant contributions in such areas of theoretical and applied science as interaction of elementary particles at superhigh energies, elementary particle physics, charged particle interactions with matter, physics of semiconductors and semiconductor devices, physics of condensed matter, radiophysics and radioelectronics, optics and quantum electronics, quantum size effects, nanophysics, sensorics, and superconductivity.
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