在单分子水平检测霍夫迈斯特效应

IF 1.8 4区物理与天体物理 Q2 PHYSICS, MULTIDISCIPLINARY

EPL Pub Date : 2023-12-04 DOI:10.1209/0295-5075/ad121b

Guangzhou Qu, Ting Liang, Yuyu Feng, Jiaen Li, Zhouhui Deng, Hu Chen, Yanhui Liu

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引用次数: 0

摘要

摘要金属阳离子对核酸的功能至关重要。鉴于金属阳离子对DNA过卷和DNA发夹折叠/解折过程以及DNA扭曲的效率，我们用磁力镊子操纵DNA发夹构建体，系统地研究了阳离子的特性以及NaCl、KCl和LiCl浓度从50 mM到300 mM的增加对折叠/解折过程的影响。在KCl、NaCl和LiCl的固定浓度下，DNA发夹解折转变的临界力和自由能的排序与{}text{Na}^{+}>{}text/{Li}^{+}$ ?>相同，并与解折速率的排序相反。这表明，与 Na+ 和 K+ 相比，溶液中加入 Li+ 能明显提高 DNA 发夹的稳定性，它们对 DNA 发夹稳定性的影响依次为 .

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Detection of Hofmeister effects at a single-molecule level

Abstract Metal cations are essential for the function of nucleic acids. Given their efficiency for DNA overwinding and the DNA hairpin folding/unfolding process coupled with DNA twist, we systematically investigated the effects of cation identities and increasing concentrations of NaCl, KCl, and LiCl from 50 mM to 300 mM on the folding/unfolding process by manipulating a DNA hairpin construct with magnetic tweezers. The critical forces and free energy of the unfolding transition of the DNA hairpin at a fixed concentration of KCl, NaCl, and LiCl followed the same ranking as

, and are reversed with the unfolding rate ranking of

{}\text{Na}^{+}>{}\text{Li}^{+}$ ?>

. This highlights that the addition of Li+ in solution obviously enhances the stability of the DNA hairpin when compared to Na+, and K+, and their efficiency on the stability of the DNA hairpin was ranked as

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来源期刊

EPL 物理-物理：综合

CiteScore

3.30

自引率

5.60%

发文量

332

审稿时长

1.9 months

期刊介绍： General physics – physics of elementary particles and fields – nuclear physics – atomic, molecular and optical physics – classical areas of phenomenology – physics of gases, plasmas and electrical discharges – condensed matter – cross-disciplinary physics and related areas of science and technology. Letters submitted to EPL should contain new results, ideas, concepts, experimental methods, theoretical treatments, including those with application potential and be of broad interest and importance to one or several sections of the physics community. The presentation should satisfy the specialist, yet remain understandable to the researchers in other fields through a suitable, clearly written introduction and conclusion (if appropriate). EPL also publishes Comments on Letters previously published in the Journal.