黑犀牛基因组的 ONT 读取组装。

IF 1.9 Q3 GENETICS & HEREDITY
Ken Kraaijeveld, Koen Bossers, Nikola Petrusevski, Stef Pieterman, Linda G R Bruins-van Sonsbeek, Floyd Wittink
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引用次数: 0

摘要

目标:黑犀牛(Diceros bicornis)是一种濒临灭绝的哺乳动物,人工繁殖计划是其保护工作的一部分。动物园中的黑犀牛通常患有慢性感染和血色素沉着病。此外,雄性繁殖力低也阻碍了黑犀牛的繁殖。为了帮助研究这些问题的研究项目,我们仅使用 ONT 测序数据对圈养雄性黑犀牛的基因组进行了测序和组装:这项工作从全血中产生了超过 100 Gb 的全基因组测序读数。这些数据被组装成一个 2.47 Gb 的基因组草案,其中包括 834 个等位基因,N50 为 29.53 Mb。基因组注释是从现有的黑犀牛基因组注释中提取的,从而检索到 99% 以上的基因特征。这一新的基因组组装将成为该物种保护基因研究的宝贵资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ONT read assembly of the black rhino genome.

Objectives: The black rhinoceros (Diceros bicornis) is an endangered mammal for which a captive breeding program is part of the conservation effort. Black rhinos in zoo's often suffer from chronic infections and heamochromatosis. Furthermore, breeding is hampered by low male fertility. To aid a research project studying these topics, we sequenced and assembled the genome of a captive male black rhino using ONT sequencing data only.

Data description: This work produced over 100 Gb whole genome sequencing reads from whole blood. These were assembled into a 2.47 Gb draft genome consisting of 834 contigs with an N50 of 29.53 Mb. The genome annotation was lifted over from an available genome annotation for black rhino, which resulted in the retrieval of over 99% of gene features. This new genome assembly will be a valuable resource in for conservation genetic research in this species.

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