空间神经脂质组学--脑部病变中脂质的MALDI质谱成像。

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS
Durga Jha, Kaj Blennow, Henrik Zetterberg, Jeffrey N. Savas, Jörg Hanrieder
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引用次数: 0

摘要

鉴于神经组织的复杂性,了解神经化学病理生理学对生物分析技术的特异性和灵敏度提出了很高的要求。质谱成像(MSI)已发展成为生物和转化研究中空间生物学的重要生化成像技术。该技术有助于全面、灵敏地阐明药物、脂质、肽和小蛋白质在原位的空间分布模式。基于基质辅助激光解吸电离(MALDI)的 MSI 是最主要的模式,因为它具有广泛的适用性,并在选择性、灵敏度、价格、通量和易用性等方面进行了合理的折衷。这对于分析空间脂质模式尤为重要,因为目前还没有其他类似的空间分析工具。因此,了解神经组织中的空间脂质生物学特性是 MSI 研究的一个关键和新兴的应用领域。本综述旨在简要介绍中枢神经系统(CNS)组织中脂质成像的 MSI 工作流程,以及开发和优化 MSI 检测时需要考虑的基本参数。此外,本综述还概述了基于 MALDI MSI 的空间神经脂质组学在绘制神经元结构脂质动态图方面的主要发展和应用,最终有助于更好地理解神经退行性疾病的病理。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Spatial neurolipidomics—MALDI mass spectrometry imaging of lipids in brain pathologies

Spatial neurolipidomics—MALDI mass spectrometry imaging of lipids in brain pathologies

Given the complexity of nervous tissues, understanding neurochemical pathophysiology puts high demands on bioanalytical techniques with respect to specificity and sensitivity. Mass spectrometry imaging (MSI) has evolved to become an important, biochemical imaging technology for spatial biology in biological and translational research. The technique facilitates comprehensive, sensitive elucidation of the spatial distribution patterns of drugs, lipids, peptides, and small proteins in situ. Matrix-assisted laser desorption ionization (MALDI)-based MSI is the dominating modality due to its broad applicability and fair compromise of selectivity, sensitivity price, throughput, and ease of use. This is particularly relevant for the analysis of spatial lipid patterns, where no other comparable spatial profiling tools are available. Understanding spatial lipid biology in nervous tissue is therefore a key and emerging application area of MSI research. The aim of this review is to give a concise guide through the MSI workflow for lipid imaging in central nervous system (CNS) tissues and essential parameters to consider while developing and optimizing MSI assays. Further, this review provides a broad overview of key developments and applications of MALDI MSI-based spatial neurolipidomics to map lipid dynamics in neuronal structures, ultimately contributing to a better understanding of neurodegenerative disease pathology.

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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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