[记录和识别闰层细胞中的去极化激活电流]。

Q3 Medicine
生理学报 Pub Date : 2024-02-25
Wen-Sen Shi, Zheng Ding, Qi Sun, Xin-Peng Duan, Cheng-Biao Zhang
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引用次数: 0

摘要

首次检测了远端肾小球闰层细胞的去极化激活电流,并根据电生理和药理特性确定了介导该电流的离子通道类型。利用Axon MultiClamp 700B贴片钳系统记录了C57BL/6J小鼠肾远端肾小球的全细胞电流,并观察了几种K+通道抑制剂对髓间细胞去极化激活电流的影响。此外,还利用免疫荧光技术研究了该通道在闰层细胞中的定位。结果表明,当浴液中的K+浓度等于细胞内液(140 mmol/L K+)时,夹层细胞中能记录到去极化激活电流,但在主细胞中却观察不到这种电流。在夹层细胞中检测到的去极化激活电流可被 Kv4.1 抑制剂阻断。免疫荧光实验表明,Kv4.1 蛋白的荧光只出现在夹层细胞中,而主细胞中没有观察到。Kv4.1蛋白的免疫荧光在夹层细胞的腔膜和基底侧膜上均可观察到,但腔膜的荧光强度高于基底侧膜。我们的结论是,在闰层细胞中检测到的去极化激活电流是由 Kv4.1 介导的,而且该通道主要表达于闰层细胞的腔膜。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Recording and identification of depolarization-activated current in intercalated cells].

The depolarization-activated current of intercalated cells in the distal nephron was detected for the first time, and the type of ion channel mediating the current was identified based on electrophysiological and pharmacological properties. The whole-cell current of distal nephron in kidney of C57BL/6J mice was recorded by Axon MultiClamp 700B patch-clamp system, and the effects of several K+ channel inhibitors on the depolarization-activated current in intercalated cells were observed. In addition, the immunofluorescence technique was used to investigate the localization of the channel in intercalated cells. The results showed that when K+ concentration of the bath solution was equal to intracellular fluid (140 mmol/L K+), the depolarization-activated current could be recorded in intercalated cells, but this current was not observed in the principal cells. The depolarization-activated current detected in the intercalated cells could be blocked by Kv4.1 inhibitors. The immunofluorescence experiment showed that the fluorescence of Kv4.1 protein was only present in intercalated cells and not observed in principal cells. Kv4.1 protein immunofluorescence was observed in the luminal and basolateral membrane of intercalated cells, but the fluorescence intensity of luminal membrane was higher than that of basolateral membrane. We conclude that the depolarization-activated current detected in intercalated cells is mediated by Kv4.1 and this channel is mainly expressed in the luminal membrane of intercalated cells.

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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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