揭示马尾藻抗水螅藻白斑综合征病毒的功效：一种体内和实验室方法

IF 1.4 4区农林科学 Q3 FISHERIES

Fisheries Science Pub Date : 2024-03-02 DOI:10.1007/s12562-024-01758-1

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引用次数: 0

摘要

摘要白斑综合征病毒（WSSV）给对虾养殖业造成了巨大的经济损失。在病毒附着过程中与宿主细胞相互作用的包膜蛋白是药物开发的潜在目标。本研究利用体外和体内分析评估了马尾藻 Wightii 的抗病毒活性。给螃蟹注射马尾藻提取物和 WSSV，并在感染后观察 30 天。抗病毒活性通过生物测定、组织病理学和体内分析得到了证实。气相色谱-质谱（GC-MS）分析确定了 S. wightii 中的 15 种化合物，硅内对接显示 VP28 和 VP26 包膜蛋白与石杉碱甲-4,22-二烯-3-酮之间有很强的相互作用。分子模拟验证了蛋白质与配体结合的稳定性。这些发现证实了 S. wightii 治疗 WSSV 的潜力。

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Unraveling the effectiveness of Sargassum wightii against white spot syndrome virus in Paratelphusa hydrodomous: an in-vivo and in-silico approach

Abstract

White spot syndrome virus (WSSV) is causing significant economic losses in shrimp farming. Envelope proteins, which interact with host cells during viral attachment, are potential targets for drug development. In this study, the antiviral activity of Sargassum wightii was assessed using in-vitro and in-silico analyses. Crabs were injected with S. wightii extract and WSSV and were observed for 30 days post-infection. The antiviral activity was confirmed by bio-assay, histopathology, and in-silico analysis. GC–MS analysis identified 15 compounds in S. wightii, and in-silico docking revealed a strong interaction between VP28 and VP26 envelope proteins and stigmasta-4,22-dien-3-one. Molecular simulations validated the stability of the protein–ligand binding. These findings confirm the potential of S. wightii as a treatment for WSSV.

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来源期刊

Fisheries Science 农林科学-渔业

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

12-24 weeks

期刊介绍： Fisheries Science is the official journal of the Japanese Society of Fisheries Science, which was established in 1932. Recognized as a leading journal in its field, Fisheries Science is respected internationally for the publication of basic and applied research articles in a broad range of subject areas relevant to fisheries science. All articles are peer-reviewed by at least two experts in the field of the submitted paper. Published six times per year, Fisheries Science includes about 120 articles per volume. It has a rich history of publishing quality papers in fisheries, biology, aquaculture, environment, chemistry and biochemistry, food science and technology, and Social Science.