牙周致病菌牙周特雷庞氏菌中 c-di-AMP 信号的特征。

IF 2.8 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Molecular Oral Microbiology Pub Date : 2024-10-01 Epub Date: 2024-03-04 DOI:10.1111/omi.12458
Aidan D Moylan, Dhara T Patel, Claire O'Brien, Edward J A Schuler, Annie N Hinson, Richard T Marconi, Daniel P Miller
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引用次数: 0

摘要

与牙周炎有关的病原菌,如牙龈特雷波纳菌,必须拥有众多的感觉传导系统,才能适应高度动态的龈下环境。迄今为止,牙周特雷波纳菌快速感知和响应环境刺激的信号通路主要尚不清楚。双(3'-5')环二腺苷单磷酸(c-di-AMP)是一种核苷酸次级信使,在许多细菌中调节渗透溶质转运、中枢代谢、生物膜发育和致病性,但在牙周尖吻畸形中却未得到描述。在这里,我们研究了牙孢子菌中的 c-di-AMP 信号转导,以了解它如何促进牙孢子菌的生理机能。我们证明了牙原蛛会产生 c-di-AMP,并鉴定了在 c-di-AMP 合成(TDE1909)和水解(TDE0027)过程中发挥作用的酶。为了研究 c-di-AMP 如何影响牙齿畸形病毒的细胞过程,进行了一项筛选试验,以确定推定的 c-di-AMP 受体蛋白。这种方法发现了 TDE0087,它被注释为一种钾摄取蛋白,是第一个与 T. denticola c-di-AMP 结合的蛋白。由于钾的平衡对维持张力压力至关重要,我们证明了牙鲆 c-di-AMP 的浓度受渗透压的影响,这表明 c-di-AMP 在低渗透溶液中对钾的吸收有负面调节作用。总之,本研究证明了牙鲆利用了 c-di-AMP 信号转导,确定了 c-di-AMP 代谢蛋白,确定了可能的受体蛋白,并将 c-di-AMP 信号转导与渗透调节联系起来。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of c-di-AMP signaling in the periodontal pathobiont, Treponema denticola.

Pathobionts associated with periodontitis, such as Treponema denticola, must possess numerous sensory transduction systems to adapt to the highly dynamic subgingival environment. To date, the signaling pathways utilized by T. denticola to rapidly sense and respond to environmental stimuli are mainly unknown. Bis-(3'-5') cyclic diadenosine monophosphate (c-di-AMP) is a nucleotide secondary messenger that regulates osmolyte transport, central metabolism, biofilm development, and pathogenicity in many bacteria but is uncharacterized in T. denticola. Here, we studied c-di-AMP signaling in T. denticola to understand how it contributes to T. denticola physiology. We demonstrated that T. denticola produces c-di-AMP and identified enzymes that function in the synthesis (TDE1909) and hydrolysis (TDE0027) of c-di-AMP. To investigate how c-di-AMP may impact T. denticola cellular processes, a screening assay was performed to identify putative c-di-AMP receptor proteins. This approach identified TDE0087, annotated as a potassium uptake protein, as the first T. denticola c-di-AMP binding protein. As potassium homeostasis is critical for maintaining turgor pressure, we demonstrated that T. denticola c-di-AMP concentrations are impacted by osmolarity, suggesting that c-di-AMP negatively regulates potassium uptake in hypoosmotic solutions. Collectively, this study demonstrates T. denticola utilizes c-di-AMP signaling, identifies c-di-AMP metabolism proteins, identifies putative receptor proteins, and correlates c-di-AMP signaling to osmoregulation.

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来源期刊
Molecular Oral Microbiology
Molecular Oral Microbiology DENTISTRY, ORAL SURGERY & MEDICINE-MICROBIOLOGY
CiteScore
6.50
自引率
5.40%
发文量
46
审稿时长
>12 weeks
期刊介绍: Molecular Oral Microbiology publishes high quality research papers and reviews on fundamental or applied molecular studies of microorganisms of the oral cavity and respiratory tract, host-microbe interactions, cellular microbiology, molecular ecology, and immunological studies of oral and respiratory tract infections. Papers describing work in virology, or in immunology unrelated to microbial colonization or infection, will not be acceptable. Studies of the prevalence of organisms or of antimicrobials agents also are not within the scope of the journal. The journal does not publish Short Communications or Letters to the Editor. Molecular Oral Microbiology is published bimonthly.
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