Marie-Charlotte Trojani, Arnaud Clavé, Isabelle Bereder, Olivier Camuzard, Régis Bernard De Dompsure, Jean-François Gonzalez, Christophe Trojani, Sabine Santucci-Darmanin, Georges F Carle, Véronique Breuil, Valérie Pierrefite-Carle
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引用次数: 0
摘要
背景：骨质疏松症（OP）是一种以骨质脆弱为特征的病理现象，影响着30%的绝经后妇女，主要原因是雌激素缺乏和氧化应激增加。人们怀疑自噬参与了 OP 的发病机制，但仍有待在人体中获得确证：方法：采用非常严格的排除标准，将因股骨颈骨折（OP 组）或全髋关节置换术（对照组）住院的绝经后妇女纳入研究。采用 Western 印迹法分析自噬水平：结果：与对照组相比，OP 患者骨中自噬体标记物 LC3-II 蛋白表达水平明显下降。此外，OP组骨骼中激素上调神经相关激酶（HUNK）的蛋白表达也明显降低：这些结果首次证明绝经后OP患者的骨自噬水平存在缺陷，并提示HUNK可能是连接雌激素流失和自噬下降的因素：临床试验注册号：ClinicalTrials.gov Identifier：NCT03175874, 2/6/2017.
Autophagy markers are decreased in bone of osteoporotic patients: a monocentric comparative study.
Background: Osteoporosis (OP) is a pathology characterized by bone fragility affecting 30% of postmenopausal women, mainly due to estrogen deprivation and increased oxidative stress. An autophagy involvement is suspected in OP pathogenesis but a definitive proof in humans remains to be obtained.
Methods: Postmenopausal women hospitalized for femoral neck fracture (OP group) or total hip replacement (Control group) were enrolled using very strict exclusion criteria. Western blot was used to analyze autophagy level.
Results: The protein expression level of the autophagosome marker LC3-II was significantly decreased in bone of OP patients relative to the control group. In addition, the protein expression of the hormonally upregulated neu-associated kinase (HUNK), which is upregulated by female hormones and promotes autophagy, was also significantly reduced in bone of the OP group.
Conclusions: These results demonstrate for the first time that postmenopausal OP patients have a deficit in bone autophagy level and suggest that HUNK could be the factor linking estrogen loss and autophagy decline.
期刊介绍：
European Journal of Endocrinology is the official journal of the European Society of Endocrinology. Its predecessor journal is Acta Endocrinologica.
The journal publishes high-quality original clinical and translational research papers and reviews in paediatric and adult endocrinology, as well as clinical practice guidelines, position statements and debates. Case reports will only be considered if they represent exceptional insights or advances in clinical endocrinology.
Topics covered include, but are not limited to, Adrenal and Steroid, Bone and Mineral Metabolism, Hormones and Cancer, Pituitary and Hypothalamus, Thyroid and Reproduction. In the field of Diabetes, Obesity and Metabolism we welcome manuscripts addressing endocrine mechanisms of disease and its complications, management of obesity/diabetes in the context of other endocrine conditions, or aspects of complex disease management. Reports may encompass natural history studies, mechanistic studies, or clinical trials.
Equal consideration is given to all manuscripts in English from any country.
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