Afnan Fatani , Xiangyang Wu , Yayra Gbotsyo , Thomas H. MacRae , Xiaojun Song , Jiabo Tan
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RNA interference was then employed to characterize ArHsp90 in <em>A. franciscana</em> nauplii and cysts. The partial knockdown of ArHsp90 slowed the development of nauplius-destined, but not cyst-destined embryos. ArHsp90 knockdown also reduced the survival and stress tolerance of nauplii newly released from <em>A. franciscana</em> females. Although the reduction of ArHsp90 had no effect on the development of diapause-destined embryos, the resulting cysts displayed reduced tolerance to desiccation and low temperature, two stresses normally encountered by <em>A. franciscana</em> in its natural environment. 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引用次数: 0
摘要
嗜极甲壳类动物Artemia franciscana的雌性会通过卵胎生途径释放活动的甲壳仔鱼,或通过卵生途径释放包囊胚胎(囊胚)。囊胚中含有大量不依赖于 ATP 的小型热休克蛋白,这种蛋白有助于应激耐受性和胚胎发育,但人们对依赖于 ATP 的分子伴侣 Hsp90 在这两个过程中的作用知之甚少。在这项研究中,从 A. franciscana 克隆了一种 Hsp90。根据推导出的氨基酸序列模拟了 ArHsp90 的特征结构域,并比对了 ArHsp90 和不同类群生物 Hsp90s 的三维结构。然后利用 RNA 干扰(RNAi)技术研究了法氏金鱼稚鱼和囊虫中 ArHsp90 的特征。部分敲除 ArHsp90 会减缓稚虫胚胎的发育,但不会减缓囊胚的发育。ArHsp90 的敲除还降低了刚从法氏无须鳕雌体中释放出来的稚虫的存活率和应激耐受性。虽然 ArHsp90 的减少不会影响囊胚的发育,但囊胚对干燥和低温的耐受性却有所降低,而这两种应激是法氏囊鲤在自然环境中通常会遇到的。研究结果表明,Hsp90 有助于法氏鲟的发育、生长和抗应激能力,而法氏鲟是一种在水产养殖中具有重要实际意义的饲料来源。
ArHsp90 is important in stress tolerance and embryo development of the brine shrimp, Artemia franciscana
Females of the extremophile crustacean, Artemia franciscana, either release motile nauplii via the ovoviviparous pathway or encysted embryos (cysts) via the oviparous pathway. Cysts contain an abundant amount of the ATP-independent small heat shock protein that contributes to stress tolerance and embryo development, however, little is known of the role of ATP-dependent molecular chaperone, heat shock protein 90 (Hsp90) in the two processes. In this study, a hsp90 was cloned from A. franciscana. Characteristic domains of ArHsp90 were simulated from the deduced amino acid sequence, and 3D structures of ArHsp90 and Hsp90s of organisms from different groups were aligned. RNA interference was then employed to characterize ArHsp90 in A. franciscana nauplii and cysts. The partial knockdown of ArHsp90 slowed the development of nauplius-destined, but not cyst-destined embryos. ArHsp90 knockdown also reduced the survival and stress tolerance of nauplii newly released from A. franciscana females. Although the reduction of ArHsp90 had no effect on the development of diapause-destined embryos, the resulting cysts displayed reduced tolerance to desiccation and low temperature, two stresses normally encountered by A. franciscana in its natural environment. The results reveal that Hsp90 contributes to the development, growth, and stress tolerance of A. franciscana, an organism of practical importance as a feed source in aquaculture.
期刊介绍:
Cell Stress and Chaperones is an integrative journal that bridges the gap between laboratory model systems and natural populations. The journal captures the eclectic spirit of the cellular stress response field in a single, concentrated source of current information. Major emphasis is placed on the effects of climate change on individual species in the natural environment and their capacity to adapt. This emphasis expands our focus on stress biology and medicine by linking climate change effects to research on cellular stress responses of animals, micro-organisms and plants.