Ting Li, Meng Wu, Junjian Tian, Yitong Li, Zhigang Li
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Specifically, the effects of EA on BAT thermogenesis were analyzed by infrared thermography, western blotting, and real-time polymerase chain reaction (PCR).</p><p><strong>Methods: </strong>: A total of 12 C57BL/6J mice were randomly divided into the EA and control groups. The EA group received EA at GV20 and EX-HN3 for 20 min once daily for 14 days. The control group underwent the same procedure but without EA. The core body temperature was monitored. Infrared thermal images of the back of each mouse in both groups were captured. BAT samples were collected after euthanasia to analyze UCP1 protein and UCP1 mRNA.</p><p><strong>Results: </strong>: The average skin temperature in the scapular region of the EA group was increased by 1.1℃ compared with that of the C group (<i>p</i> < 0.05). Additionally, the average temperature along the governor vessel in the EA group was increased by 1.6℃ (<i>p</i> = 0.045). EA significantly increased the expression of UCP1 protein (<i>p</i> = 0.001) and UCP1 mRNA (<i>p</i> = 0.002) in BAT, suggesting a potential link between EA and BAT thermogenesis.</p><p><strong>Conclusion: </strong>: EA induced BAT thermogenesis, suggesting GV20 and EX-HN3 as potential acupoints for BAT stimulation. The experimental results also highlighted unique meridian characteristics as demonstrated by elevated skin temperature along the governor vessel in mice.</p>","PeriodicalId":46854,"journal":{"name":"Journal of Acupuncture and Meridian Studies","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effect of Electroacupuncture Stimulation on Brown Adipose Tissue Thermogenesis.\",\"authors\":\"Ting Li, Meng Wu, Junjian Tian, Yitong Li, Zhigang Li\",\"doi\":\"10.51507/j.jams.2024.17.1.1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>: Brown adipose tissue (BAT) is a unique thermogenic tissue in mammals mediated by uncoupling protein 1 (UCP1). The energy generated by glucose and triglyceride metabolism is released and transmitted throughout the body as heat. Understanding the factors influencing BAT function is crucial to determine its metabolic significance and effects on overall health. Although studies have shown that electroacupuncture (EA) at specific acupoints (e.g., ST36) can stimulate BAT, its effects at other acupoints are not well understood. Further research is needed to investigate the potential effects of EA at these acupoints and their association with BAT activation.</p><p><strong>Objectives: </strong>: This study aimed to investigate the effects of EA at the GV20 and EX-HN3 acupoints. Specifically, the effects of EA on BAT thermogenesis were analyzed by infrared thermography, western blotting, and real-time polymerase chain reaction (PCR).</p><p><strong>Methods: </strong>: A total of 12 C57BL/6J mice were randomly divided into the EA and control groups. The EA group received EA at GV20 and EX-HN3 for 20 min once daily for 14 days. The control group underwent the same procedure but without EA. The core body temperature was monitored. Infrared thermal images of the back of each mouse in both groups were captured. BAT samples were collected after euthanasia to analyze UCP1 protein and UCP1 mRNA.</p><p><strong>Results: </strong>: The average skin temperature in the scapular region of the EA group was increased by 1.1℃ compared with that of the C group (<i>p</i> < 0.05). Additionally, the average temperature along the governor vessel in the EA group was increased by 1.6℃ (<i>p</i> = 0.045). EA significantly increased the expression of UCP1 protein (<i>p</i> = 0.001) and UCP1 mRNA (<i>p</i> = 0.002) in BAT, suggesting a potential link between EA and BAT thermogenesis.</p><p><strong>Conclusion: </strong>: EA induced BAT thermogenesis, suggesting GV20 and EX-HN3 as potential acupoints for BAT stimulation. The experimental results also highlighted unique meridian characteristics as demonstrated by elevated skin temperature along the governor vessel in mice.</p>\",\"PeriodicalId\":46854,\"journal\":{\"name\":\"Journal of Acupuncture and Meridian Studies\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-02-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Acupuncture and Meridian Studies\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.51507/j.jams.2024.17.1.1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Acupuncture and Meridian Studies","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.51507/j.jams.2024.17.1.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 0
摘要
背景： ：棕色脂肪组织（BAT）是哺乳动物特有的产热组织，由解偶联蛋白 1（UCP1）介导。葡萄糖和甘油三酯代谢产生的能量以热量的形式释放并传遍全身。了解影响 BAT 功能的因素对于确定其代谢意义和对整体健康的影响至关重要。尽管有研究表明，在特定穴位（如 ST36）进行电针（EA）可以刺激 BAT，但对其在其他穴位的作用还不甚了解。需要进一步研究电针在这些穴位的潜在作用及其与 BAT 激活的关联：本研究旨在探讨 EA 对 GV20 和 EX-HN3 穴位的影响。具体而言，通过红外热成像、Western 印迹和实时聚合酶链反应（PCR）分析 EA 对 BAT 产热的影响：将 12 只 C57BL/6J 小鼠随机分为 EA 组和对照组。EA组在GV20和EX-HN3处接受EA，每天一次，每次20分钟，共14天。对照组接受同样的治疗，但不注射 EA。监测核心体温。捕捉两组小鼠背部的红外热图像。安乐死后采集 BAT 样本，分析 UCP1 蛋白和 UCP1 mRNA：EA组肩胛区的平均皮肤温度比C组升高了1.1℃（p < 0.05）。此外，EA 组沿督血管的平均温度升高了 1.6℃（p = 0.045）。EA 能明显增加 BAT 中 UCP1 蛋白（p = 0.001）和 UCP1 mRNA（p = 0.002）的表达，表明 EA 与 BAT 产热之间存在潜在联系：结论：EA诱导BAT产热，表明GV20和EX-HN3是刺激BAT的潜在穴位。实验结果还强调了小鼠督脉沿线皮肤温度升高的独特经络特征。
Effect of Electroacupuncture Stimulation on Brown Adipose Tissue Thermogenesis.
Background: : Brown adipose tissue (BAT) is a unique thermogenic tissue in mammals mediated by uncoupling protein 1 (UCP1). The energy generated by glucose and triglyceride metabolism is released and transmitted throughout the body as heat. Understanding the factors influencing BAT function is crucial to determine its metabolic significance and effects on overall health. Although studies have shown that electroacupuncture (EA) at specific acupoints (e.g., ST36) can stimulate BAT, its effects at other acupoints are not well understood. Further research is needed to investigate the potential effects of EA at these acupoints and their association with BAT activation.
Objectives: : This study aimed to investigate the effects of EA at the GV20 and EX-HN3 acupoints. Specifically, the effects of EA on BAT thermogenesis were analyzed by infrared thermography, western blotting, and real-time polymerase chain reaction (PCR).
Methods: : A total of 12 C57BL/6J mice were randomly divided into the EA and control groups. The EA group received EA at GV20 and EX-HN3 for 20 min once daily for 14 days. The control group underwent the same procedure but without EA. The core body temperature was monitored. Infrared thermal images of the back of each mouse in both groups were captured. BAT samples were collected after euthanasia to analyze UCP1 protein and UCP1 mRNA.
Results: : The average skin temperature in the scapular region of the EA group was increased by 1.1℃ compared with that of the C group (p < 0.05). Additionally, the average temperature along the governor vessel in the EA group was increased by 1.6℃ (p = 0.045). EA significantly increased the expression of UCP1 protein (p = 0.001) and UCP1 mRNA (p = 0.002) in BAT, suggesting a potential link between EA and BAT thermogenesis.
Conclusion: : EA induced BAT thermogenesis, suggesting GV20 and EX-HN3 as potential acupoints for BAT stimulation. The experimental results also highlighted unique meridian characteristics as demonstrated by elevated skin temperature along the governor vessel in mice.
期刊介绍：
The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine. Manuscripts should fall into one of the categories: topical review, original research paper, brief ... click here for full Aims & Scope The Journal of Acupuncture and Meridian Studies is a quarterly, peer-reviewed journal featuring high-quality studies related to basic and clinical acupuncture and meridian research. It also includes new paradigm of integrative research, covering East–West and traditional–modern medicine.
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