{"title":"子痫前期 HMOX1 DNA 甲基化和基因表达的研究以及 miR-153-3p 的诊断潜力。","authors":"Somayeh Rahimi, Nayebali Rezvani, Saeed Khazayel, Nazanin Jalilian, Ebrahim Shakiba, Fatemeh Khadir, Kheirollah Yari, Zohreh Rahimi","doi":"10.2217/epi-2023-0377","DOIUrl":null,"url":null,"abstract":"<p><p><b>Background:</b> The objective was to elucidate the potential epigenetic regulatory mechanism in <i>HMOX1</i> expression in preeclampsia. <b>Materials & methods:</b> <i>HMOX1</i> promoter DNA methylation was evaluated in the placental tissue and blood of preeclamptic and normotensive pregnant women. <i>HMOX1</i> and <i>miR-153-3p</i> gene expression were assessed in placental tissue and peripheral blood mononuclear cells (PBMCs). Related microarray datasets in the Gene Expression Omnibus database were also analyzed. <b>Results:</b> In placental tissue, despite <i>HMOX1</i> expression downregulation, there was no significant change in <i>HMOX1</i> methylation. In PBMCs, there was no significant alteration in <i>HMOX1</i> expression, while hypomethylation was observed in blood. The <i>miR-153-3p</i> expression increased in the placental tissue and in the PBMCs of preeclampsia. <b>Conclusion:</b> DNA methylation does not affect <i>HMOX1</i> expression, while <i>miR-153-3p</i> might be a biomarker for preeclampsia.</p>","PeriodicalId":11959,"journal":{"name":"Epigenomics","volume":" ","pages":"389-401"},"PeriodicalIF":3.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The study of <i>HMOX1</i> DNA methylation and gene expression and the diagnostic potential of <i>miR-153-3p</i> in preeclampsia.\",\"authors\":\"Somayeh Rahimi, Nayebali Rezvani, Saeed Khazayel, Nazanin Jalilian, Ebrahim Shakiba, Fatemeh Khadir, Kheirollah Yari, Zohreh Rahimi\",\"doi\":\"10.2217/epi-2023-0377\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Background:</b> The objective was to elucidate the potential epigenetic regulatory mechanism in <i>HMOX1</i> expression in preeclampsia. <b>Materials & methods:</b> <i>HMOX1</i> promoter DNA methylation was evaluated in the placental tissue and blood of preeclamptic and normotensive pregnant women. <i>HMOX1</i> and <i>miR-153-3p</i> gene expression were assessed in placental tissue and peripheral blood mononuclear cells (PBMCs). Related microarray datasets in the Gene Expression Omnibus database were also analyzed. <b>Results:</b> In placental tissue, despite <i>HMOX1</i> expression downregulation, there was no significant change in <i>HMOX1</i> methylation. In PBMCs, there was no significant alteration in <i>HMOX1</i> expression, while hypomethylation was observed in blood. The <i>miR-153-3p</i> expression increased in the placental tissue and in the PBMCs of preeclampsia. <b>Conclusion:</b> DNA methylation does not affect <i>HMOX1</i> expression, while <i>miR-153-3p</i> might be a biomarker for preeclampsia.</p>\",\"PeriodicalId\":11959,\"journal\":{\"name\":\"Epigenomics\",\"volume\":\" \",\"pages\":\"389-401\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Epigenomics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2217/epi-2023-0377\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2217/epi-2023-0377","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/27 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
The study of HMOX1 DNA methylation and gene expression and the diagnostic potential of miR-153-3p in preeclampsia.
Background: The objective was to elucidate the potential epigenetic regulatory mechanism in HMOX1 expression in preeclampsia. Materials & methods:HMOX1 promoter DNA methylation was evaluated in the placental tissue and blood of preeclamptic and normotensive pregnant women. HMOX1 and miR-153-3p gene expression were assessed in placental tissue and peripheral blood mononuclear cells (PBMCs). Related microarray datasets in the Gene Expression Omnibus database were also analyzed. Results: In placental tissue, despite HMOX1 expression downregulation, there was no significant change in HMOX1 methylation. In PBMCs, there was no significant alteration in HMOX1 expression, while hypomethylation was observed in blood. The miR-153-3p expression increased in the placental tissue and in the PBMCs of preeclampsia. Conclusion: DNA methylation does not affect HMOX1 expression, while miR-153-3p might be a biomarker for preeclampsia.
期刊介绍：
Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community.
Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
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