{"title":"hsa_circ_0087100/hsa-miR-6743-5p 通过调节 STAT1 影响糖尿病视网膜病变中 Th1 细胞的分化。","authors":"Shuai He, Dongwei Lai, Chenggong Ma, Chunren Meng, Chunyang Cai, Qian Chen, Chufeng Gu, Qinghua Qiu","doi":"10.2217/epi-2023-0359","DOIUrl":null,"url":null,"abstract":"<p><p><b>Objective:</b> To elucidate the role of the competitive endogenous RNA (ceRNA) network in immune infiltration of diabetic retinopathy (DR). <b>Methods:</b> We obtained differentially expressed (DE) circRNAs, miRNAs and mRNAs from the Gene Expression Omnibus database. Then, we identified immune infiltration by CIBERSORT and single-sample gene set enrichment analysis and discovered co-expression genes by weighted gene co-expression network analysis. Furthermore, <i>STAT1</i>-mediated Th1 differentiation was determined in DR cell models, DR patients and DR mouse models. <b>Results:</b> <i>hsa_circ_0087100/hsa-miR-6743-5p/STAT1</i> was involved in immune infiltration of Th1 cells. Aberrant expression of the ceRNA network and <i>STAT1</i>-mediated Th1 differentiation was thus verified <i>in vitro</i> and <i>in vivo</i>. <b>Conclusion:</b> <i>hsa_circ_0087100/hsa-miR-6743-5p/STAT1</i> may affect Th1 cell differentiation in DR.</p>","PeriodicalId":11959,"journal":{"name":"Epigenomics","volume":" ","pages":"427-444"},"PeriodicalIF":3.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>hsa_circ_0087100/hsa-miR-6743-5p</i> affects Th1 cell differentiation by regulating <i>STAT1</i> in diabetic retinopathy.\",\"authors\":\"Shuai He, Dongwei Lai, Chenggong Ma, Chunren Meng, Chunyang Cai, Qian Chen, Chufeng Gu, Qinghua Qiu\",\"doi\":\"10.2217/epi-2023-0359\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Objective:</b> To elucidate the role of the competitive endogenous RNA (ceRNA) network in immune infiltration of diabetic retinopathy (DR). <b>Methods:</b> We obtained differentially expressed (DE) circRNAs, miRNAs and mRNAs from the Gene Expression Omnibus database. Then, we identified immune infiltration by CIBERSORT and single-sample gene set enrichment analysis and discovered co-expression genes by weighted gene co-expression network analysis. Furthermore, <i>STAT1</i>-mediated Th1 differentiation was determined in DR cell models, DR patients and DR mouse models. <b>Results:</b> <i>hsa_circ_0087100/hsa-miR-6743-5p/STAT1</i> was involved in immune infiltration of Th1 cells. Aberrant expression of the ceRNA network and <i>STAT1</i>-mediated Th1 differentiation was thus verified <i>in vitro</i> and <i>in vivo</i>. <b>Conclusion:</b> <i>hsa_circ_0087100/hsa-miR-6743-5p/STAT1</i> may affect Th1 cell differentiation in DR.</p>\",\"PeriodicalId\":11959,\"journal\":{\"name\":\"Epigenomics\",\"volume\":\" \",\"pages\":\"427-444\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Epigenomics\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2217/epi-2023-0359\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/2/27 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2217/epi-2023-0359","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/27 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
摘要
目的阐明竞争性内源性 RNA（ceRNA）网络在糖尿病视网膜病变（DR）免疫浸润中的作用。方法我们从基因表达总库（Gene Expression Omnibus）数据库中获得了差异表达（DE）的circRNA、miRNA和mRNA。然后，我们通过 CIBERSORT 和单样本基因组富集分析确定了免疫浸润，并通过加权基因共表达网络分析发现了共表达基因。此外，我们还测定了 STAT1 介导的 Th1 分化在 DR 细胞模型、DR 患者和 DR 小鼠模型中的表现。结果：hsa_circ_0087100/hsa-miR-6743-5p/STAT1参与了Th1细胞的免疫浸润。因此，ceRNA 网络的异常表达和 STAT1 介导的 Th1 分化在体外和体内都得到了验证。结论：hsa_circ_0087100/hsa-miR-6743-5p/STAT1 可能会影响 DR 中 Th1 细胞的分化。
hsa_circ_0087100/hsa-miR-6743-5p affects Th1 cell differentiation by regulating STAT1 in diabetic retinopathy.
Objective: To elucidate the role of the competitive endogenous RNA (ceRNA) network in immune infiltration of diabetic retinopathy (DR). Methods: We obtained differentially expressed (DE) circRNAs, miRNAs and mRNAs from the Gene Expression Omnibus database. Then, we identified immune infiltration by CIBERSORT and single-sample gene set enrichment analysis and discovered co-expression genes by weighted gene co-expression network analysis. Furthermore, STAT1-mediated Th1 differentiation was determined in DR cell models, DR patients and DR mouse models. Results:hsa_circ_0087100/hsa-miR-6743-5p/STAT1 was involved in immune infiltration of Th1 cells. Aberrant expression of the ceRNA network and STAT1-mediated Th1 differentiation was thus verified in vitro and in vivo. Conclusion:hsa_circ_0087100/hsa-miR-6743-5p/STAT1 may affect Th1 cell differentiation in DR.
期刊介绍：
Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community.
Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
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