一碳代谢营养素影响肝脏中 DNA 甲基化与基因表达之间的相互作用,从而促进大西洋鲑鱼的蛋白质合成。

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Epigenetics Pub Date : 2024-12-01 Epub Date: 2024-02-25 DOI:10.1080/15592294.2024.2318517
Takaya Saito, Marit Espe, Vibeke Vikeså, Christoph Bock, Tårn H Thomsen, Anne-Catrin Adam, Jorge M O Fernandes, Kaja H Skjaerven
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引用次数: 0

摘要

补充一碳(1C)代谢微量营养素(包括 B 族维生素和蛋氨酸)对大西洋鲑(Salmo salar)的健康生长和发育至关重要。然而,最近在鲑鱼养殖中转向非鱼粉日粮,导致需要重新评估推荐的微量营养素水平。尽管 1C 代谢在生长性能和各种细胞调控中具有重要作用,但人们对这些日粮改变所影响的分子机制却不甚了解。为了研究 1C 营养素的分子效应,我们利用两种全局数据分析了基因表达和 DNA 甲基化:RNA测序(RNA-seq)和还原呈现亚硫酸氢盐测序(RRBS)。我们在喂养试验结束时收集了鱼的肝脏样本,该试验持续了 220 天,一直到蜕皮阶段,在试验过程中给鱼喂养了三种不同水平的四种关键 1C 营养物质:蛋氨酸、维生素 B6、B9 和 B12。我们的研究结果表明,1C营养素的剂量对大西洋鲑肝脏中的遗传和表观遗传调控有显著影响,尤其是在与蛋白质合成相关的生物通路中。这些途径中 DNA 甲基化与基因表达之间的相互作用可能在 1C 代谢影响生长性能的机制中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
One-carbon metabolism nutrients impact the interplay between DNA methylation and gene expression in liver, enhancing protein synthesis in Atlantic salmon.

Supplementation of one-carbon (1C) metabolism micronutrients, which include B-vitamins and methionine, is essential for the healthy growth and development of Atlantic salmon (Salmo salar). However, the recent shift towards non-fish meal diets in salmon aquaculture has led to the need for reassessments of recommended micronutrient levels. Despite the importance of 1C metabolism in growth performance and various cellular regulations, the molecular mechanisms affected by these dietary alterations are less understood. To investigate the molecular effect of 1C nutrients, we analysed gene expression and DNA methylation using two types of omics data: RNA sequencing (RNA-seq) and reduced-representation bisulphite sequencing (RRBS). We collected liver samples at the end of a feeding trial that lasted 220 days through the smoltification stage, where fish were fed three different levels of four key 1C nutrients: methionine, vitamin B6, B9, and B12. Our results indicate that the dosage of 1C nutrients significantly impacts genetic and epigenetic regulations in the liver of Atlantic salmon, particularly in biological pathways related to protein synthesis. The interplay between DNA methylation and gene expression in these pathways may play an important role in the mechanisms underlying growth performance affected by 1C metabolism.

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来源期刊
Epigenetics
Epigenetics 生物-生化与分子生物学
CiteScore
6.80
自引率
2.70%
发文量
82
审稿时长
3-8 weeks
期刊介绍: Epigenetics publishes peer-reviewed original research and review articles that provide an unprecedented forum where epigenetic mechanisms and their role in diverse biological processes can be revealed, shared, and discussed. Epigenetics research studies heritable changes in gene expression caused by mechanisms others than the modification of the DNA sequence. Epigenetics therefore plays critical roles in a variety of biological systems, diseases, and disciplines. Topics of interest include (but are not limited to): DNA methylation Nucleosome positioning and modification Gene silencing Imprinting Nuclear reprogramming Chromatin remodeling Non-coding RNA Non-histone chromosomal elements Dosage compensation Nuclear organization Epigenetic therapy and diagnostics Nutrition and environmental epigenetics Cancer epigenetics Neuroepigenetics
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