外周血单核细胞衍生的细胞外小泡对 K562 白血病细胞株的影响

Q3 Medicine

Acta medica Iranica Pub Date : 2024-02-18 DOI:10.18502/acta.v61i8.14901

H. M. valandani, R. M. Khalilabadi, Amir Mohammad Zahedi

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引用次数: 0

摘要

最近，细胞外囊泡（EVs）的能力引起了人们的极大兴趣。EVs是来源细胞和接受细胞之间通过传递信息进行细胞间交流的途径。本文研究了外周血单核细胞衍生的EVs对慢性骨髓性白血病细胞株k562增殖和凋亡的影响。采用胰蓝染色法检测细胞活力，然后用 MTT 法评估代谢活性。用 MNC 衍生的 EV 处理 k562 细胞系后，使用流式细胞计数法评估细胞凋亡和细胞周期进展。结果表明，MNC-EV 对 k562 细胞的生长和增殖没有抑制作用。我们的数据没有显示用 MNC 衍生 EV 处理 k562 细胞株后，其生长增强情况有任何明显变化。由此看来，肿瘤衍生的细胞外囊泡通过细胞外囊泡之间的交流支持了肿瘤细胞的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Effect of Extracellular Vesicles Derived From Peripheral Blood Mononuclear Cells on K562 Leukemia Cell Line

Recently, extracellular vesicles (EVs) are generating considerable interest in terms of their ability. EVs defined themselves as a route for intercellular communication between the origin cells and the recipient ones by transferring information. This paper investigates the influence of peripheral blood mononuclear cells derived EVs on proliferation and apoptosis of the chronic myelogenous leukemia cell line k562. Trypan blue staining was used to detect cell viability subsequently, metabolic activity was assessed by the MTT assay. Cell apoptosis and cell cycle progression were evaluated using flowcytometric assay after treatment of k562 cell line with MNC derived EVs. Our results showed that MNC-EVs have no inhibitory effect on k562 cell growth and proliferation. Our data did not reveal any significant variations in the case of enhancing k562 cell line growth following treatment with MNC derived EVs. It seems tumour-derived extracellular vesicles support tumour cells growth by communicating with each other through their extracellular vesicles.

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来源期刊

Acta medica Iranica Medicine-Medicine (all)

CiteScore

0.70

自引率

0.00%

发文量

审稿时长

18 weeks

期刊介绍： ACTA MEDICA IRANICA (p. ISSN 0044-6025; e. ISSN: 1735-9694) is the official journal of the Faculty of Medicine, Tehran University of Medical Sciences. The journal is the oldest scientific medical journal of the country, which has been published from 1960 onward in English language. Although it had been published quarterly in the past, the journal has been published bimonthly (6 issues per year) from the year 2004. Acta Medica Iranica it is an international journal with multidisciplinary scope which publishes original research papers, review articles, case reports, and letters to the editor from all over the world. The journal has a wide scope and allows scientists, clinicians, and academic members to publish their original works in this field.