利用骨髓移植模型探讨传统 CD8αβ+ T 细胞和 CD4+ T 细胞在肠道免疫病理中的作用

Amneh Aoudi, Ossama Labiad, Ramdane Igalouzene, Ousséma Mejri, Maxime Sanchez, Saïdi Soudja
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引用次数: 0

摘要

炎症性肠病(IBD)的特点是针对微生物群的异常免疫反应。T细胞在介导病理过程中发挥着关键作用,这一点已得到公认。为了设计出更好的治疗策略,评估每个 T 细胞亚群在介导病理过程中的贡献至关重要。本实验提出了一种在骨髓移植小鼠模型中鉴定导致肠道免疫病理的特异性效应T细胞群的方法。在这里,我们在骨髓移植小鼠模型中使用了抗CD4和抗CD8β耗竭抗体,在慢性肠道炎症遗传小鼠模型中鉴定了导致肠道损伤的效应T细胞群。主要特点 - 该方案可用于研究 CD4+ 或 CD8αβ+ 在炎症性肠病(IBD)移植模型中的作用。- 该方案可以很容易地进行调整,以研究可能在 IBD 中起作用的其他免疫细胞或分子的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Addressing the Role of Conventional CD8αβ+ T Cells and CD4+ T Cells in Intestinal Immunopathology Using a Bone Marrow–Engrafted Model
Inflammatory bowel disease (IBD) is characterized by an aberrant immune response against microbiota. It is well established that T cells play a critical role in mediating the pathology. Assessing the contribution of each subset of T cells in mediating the pathology is crucial in order to design better therapeutic strategies. This protocol presents a method to identify the specific effector T-cell population responsible for intestinal immunopathologies in bone marrow–engrafted mouse models. Here, we used anti-CD4 and anti-CD8β depleting antibodies in bone marrow–engrafted mouse models to identify the effector T-cell population responsible for intestinal damage in a genetic mouse model of chronic intestinal inflammation. Key features • This protocol allows addressing the role of CD4+ or CD8αβ+ in an engrafted model of inflammatory bowel disease (IBD). • This protocol can easily be adapted to address the role of other immune cells or molecules that may play a role in IBD.
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