Xu-Ze Zhang , Xiao-Dong Ma , Wan-Ting Wang , Fei Peng , Ye-Mao Hou , Yue-Xia Shen , Yu-Qi Sun , Jin-Fang Chen , Yi-Jin Yin , Yu-Ye Zeng , Yi Yu , Peng Zhou , Fu-Hao Zhang , Yan-Feng He , Ying-Fang Shen
{"title":"两种不同肤色的库库诺氏蛙皮肤组织学和转录组学对比分析","authors":"Xu-Ze Zhang , Xiao-Dong Ma , Wan-Ting Wang , Fei Peng , Ye-Mao Hou , Yue-Xia Shen , Yu-Qi Sun , Jin-Fang Chen , Yi-Jin Yin , Yu-Ye Zeng , Yi Yu , Peng Zhou , Fu-Hao Zhang , Yan-Feng He , Ying-Fang Shen","doi":"10.1016/j.cbd.2024.101217","DOIUrl":null,"url":null,"abstract":"<div><p>This study compares the skin structures of <em>Rana kukunoris</em> with two different skin colors living in the same area of Haibei in the Northeastern Qinghai-Tibet Plateau. The skin thickness of the khaki <em>R. kukunoris</em> was significantly greater than that of the brown <em>R. kukunoris</em> (<em>P</em> < 0.01), and significantly more mucous and granular glands were present on the dorsal skin of the khaki frog (<em>P</em> < 0.05). Meanwhile, the melanocytes on the dorsal skin of the brown frog were significantly larger than those on the khaki one (<em>P</em> < 0.05). Morphological changes in the expansion and aggregation of melanocytes seemed to deepen the skin color of <em>R. kukunoris</em>. Moreover, transcriptome sequencing identified tyrosine metabolism, melanogenesis, and riboflavin metabolism as the main pathways involved in melanin formation and metabolism in brown <em>R. kukunoris</em>. <em>TYR, MC1R</em> was upregulated as the skin color of <em>R. kukunoris</em> was deepened and contributed to melanin production and metabolism<em>.</em> In contrast, the khaki frog had significantly more upregulated genes and metabolic pathways related to autoimmunity. The khaki frog appeared to defend against ultraviolet (UV) radiation-induced damage by secreting mucus and small molecular peptides, whereas the brown frog protected itself by distributing a large amount of melanin. Hence, the different skin colors of <em>R. kukunoris</em> might represent different adaptation strategies for survival in the intense UV radiation environment of the Qinghai-Tibet Plateau.</p></div>","PeriodicalId":55235,"journal":{"name":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","volume":"50 ","pages":"Article 101217"},"PeriodicalIF":2.2000,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative skin histological and transcriptomic analysis of Rana kukunoris with two different skin colors\",\"authors\":\"Xu-Ze Zhang , Xiao-Dong Ma , Wan-Ting Wang , Fei Peng , Ye-Mao Hou , Yue-Xia Shen , Yu-Qi Sun , Jin-Fang Chen , Yi-Jin Yin , Yu-Ye Zeng , Yi Yu , Peng Zhou , Fu-Hao Zhang , Yan-Feng He , Ying-Fang Shen\",\"doi\":\"10.1016/j.cbd.2024.101217\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study compares the skin structures of <em>Rana kukunoris</em> with two different skin colors living in the same area of Haibei in the Northeastern Qinghai-Tibet Plateau. The skin thickness of the khaki <em>R. kukunoris</em> was significantly greater than that of the brown <em>R. kukunoris</em> (<em>P</em> < 0.01), and significantly more mucous and granular glands were present on the dorsal skin of the khaki frog (<em>P</em> < 0.05). Meanwhile, the melanocytes on the dorsal skin of the brown frog were significantly larger than those on the khaki one (<em>P</em> < 0.05). Morphological changes in the expansion and aggregation of melanocytes seemed to deepen the skin color of <em>R. kukunoris</em>. Moreover, transcriptome sequencing identified tyrosine metabolism, melanogenesis, and riboflavin metabolism as the main pathways involved in melanin formation and metabolism in brown <em>R. kukunoris</em>. <em>TYR, MC1R</em> was upregulated as the skin color of <em>R. kukunoris</em> was deepened and contributed to melanin production and metabolism<em>.</em> In contrast, the khaki frog had significantly more upregulated genes and metabolic pathways related to autoimmunity. The khaki frog appeared to defend against ultraviolet (UV) radiation-induced damage by secreting mucus and small molecular peptides, whereas the brown frog protected itself by distributing a large amount of melanin. Hence, the different skin colors of <em>R. kukunoris</em> might represent different adaptation strategies for survival in the intense UV radiation environment of the Qinghai-Tibet Plateau.</p></div>\",\"PeriodicalId\":55235,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology D-Genomics & Proteomics\",\"volume\":\"50 \",\"pages\":\"Article 101217\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-02-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology D-Genomics & Proteomics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1744117X24000303\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology D-Genomics & Proteomics","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1744117X24000303","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Comparative skin histological and transcriptomic analysis of Rana kukunoris with two different skin colors
This study compares the skin structures of Rana kukunoris with two different skin colors living in the same area of Haibei in the Northeastern Qinghai-Tibet Plateau. The skin thickness of the khaki R. kukunoris was significantly greater than that of the brown R. kukunoris (P < 0.01), and significantly more mucous and granular glands were present on the dorsal skin of the khaki frog (P < 0.05). Meanwhile, the melanocytes on the dorsal skin of the brown frog were significantly larger than those on the khaki one (P < 0.05). Morphological changes in the expansion and aggregation of melanocytes seemed to deepen the skin color of R. kukunoris. Moreover, transcriptome sequencing identified tyrosine metabolism, melanogenesis, and riboflavin metabolism as the main pathways involved in melanin formation and metabolism in brown R. kukunoris. TYR, MC1R was upregulated as the skin color of R. kukunoris was deepened and contributed to melanin production and metabolism. In contrast, the khaki frog had significantly more upregulated genes and metabolic pathways related to autoimmunity. The khaki frog appeared to defend against ultraviolet (UV) radiation-induced damage by secreting mucus and small molecular peptides, whereas the brown frog protected itself by distributing a large amount of melanin. Hence, the different skin colors of R. kukunoris might represent different adaptation strategies for survival in the intense UV radiation environment of the Qinghai-Tibet Plateau.
期刊介绍:
Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology.
Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.