视野测试迷宫和视觉迷宫:评估动物视野缺损的可行技术。

IF 1.3 4区 医学 Q4 PHARMACOLOGY & PHARMACY
Shivani Behera , Ashmita Das , Jaya Shree , Pranay Soni , Devi Prasad Pandey , Surendra H. Bodakhe
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引用次数: 0

摘要

青光眼引起的视野缺损是一个严重而令人担忧的问题,它会导致视力范围受限和视觉质量下降。这种视力损失的发展过程从中央旁弧形视网膜瘤开始,最终发展到后期的环形视网膜瘤和视野缩小。目前,还没有动物模型可用于筛查这种视力丧失模式。不过,我们已经成功开发出了两种评估视野缺损的迷宫--针对周边视觉缺损的视野测试迷宫(VFTZ)和针对中心视力缺损的视野迷宫(VM)。我们的研究包括使用脂多糖(LPS)诱导 Wistar 大鼠和 Sprague Dawley 大鼠患上青光眼,并在 VFTZ 和 VM 中对它们进行测试。在研究过程中,我们使用拉坦前列素和多佐胺滴眼液作为标准候选药物。我们使用眼压计、眼底镜和明暗模型技术对动物的眼压、视网膜血管成像和焦虑进行了评估。此外,我们还使用紫外光谱量化了视网膜的抗氧化参数。我们的研究结果表明,与正常或药物治疗的动物相比,VFTZ 中外周视野缺损的动物到达目标的时间明显更长,在迷宫中花费的时间也更长(P<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The visual field-testing maze and vision maze: Feasible techniques to evaluate visual field loss in animals

Visual field loss due to glaucoma is a severe and concerning problem, leading to limited visual range and poor quality vision. The progression of this loss begins with a para-central arcuate scotoma which eventually advances to a ring scotoma and constricted visual fields in later stages. Currently, no animal model is available for screening this pattern of vision loss. However, we have successfully developed two mazes to evaluate visual field loss - the visual field-testing maze (VFTZ) for peripheral vision loss and the vision maze (VM) for central vision loss. Our studies involved inducing glaucoma in Wistar and Sprague Dawley rats using lipopolysaccharide (LPS) and testing them in VFTZ and VM. We used Latanoprost and dorzolamide eye drops as standard drug candidates during the study. We evaluated the animals for intraocular pressure, retinal vasculature imaging, and anxiety using tonometry, ophthalmoscopy, and light and dark model techniques. Furthermore, we quantified the antioxidant parameters of the retina using UV spectroscopy. Our findings showed that animals with peripheral visual field loss in VFTZ took significantly more time to reach the goal and spent more time within the maze compared to normal or drug-treated animals (P < 0.001). Additionally, animals with compromised central visual field in VM spent more time in a particular arm and changed arms less frequently (P < 0.001) compared to normal or drug-treated animals. Moreover, we observed that glaucomatous rats exhibited elevated anxiety levels and impaired performance in the mazes, emphasizing the impact of vision loss on anxiety. Finally, the antioxidant and ATPase alterations in the retinal layers verified the glaucomatous changes in the experimental animals. Based on our remarkable findings, we strongly recommend the use of VFTZ and VM to evaluate visual field loss in animals.

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来源期刊
Journal of pharmacological and toxicological methods
Journal of pharmacological and toxicological methods PHARMACOLOGY & PHARMACY-TOXICOLOGY
CiteScore
3.60
自引率
10.50%
发文量
56
审稿时长
26 days
期刊介绍: Journal of Pharmacological and Toxicological Methods publishes original articles on current methods of investigation used in pharmacology and toxicology. Pharmacology and toxicology are defined in the broadest sense, referring to actions of drugs and chemicals on all living systems. With its international editorial board and noted contributors, Journal of Pharmacological and Toxicological Methods is the leading journal devoted exclusively to experimental procedures used by pharmacologists and toxicologists.
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