小鼠跨代表观遗传的遗传混淆。

IF 2.9 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Epigenetics Pub Date : 2024-12-01 Epub Date: 2024-02-18 DOI:10.1080/15592294.2024.2318519
Daniel M Sapozhnikov, Moshe Szyf
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引用次数: 0

摘要

哺乳动物的跨代表观遗传仍然是一个有争议的现象。Takahashi 等人最近的一项研究通过使用基于 CRISPR/Cas9 的表观遗传编辑技术改变特定启动子的 DNA 甲基化水平,然后证明甲基化增量在后代中的遗传性,为小鼠的这种遗传模式提供了证据。在这篇技术评论中,我们认为原始研究中使用的方法本质上放大了遗传变化的可能性,从而导致了表观遗传变化的遗传性。我们提供的证据表明,在这些实验中确实发生了多种来源的遗传变化,并探讨了这些变化可能导致表观遗传变化明显遗传的几种途径。我们的结论是,不能排除遗传的遗传基础,因此,原始研究没有充分证实表观遗传的跨代遗传性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic confounds of transgenerational epigenetic inheritance in mice.

Transgenerational epigenetic inheritance in mammals remains a controversial phenomenon. A recent study by Takahashi et al. provides evidence for this mode of inheritance in mice by using a CRISPR/Cas9-based epigenetic editing technique to modify DNA methylation levels at specific promoters and then demonstrating the inheritance of the gain in methylation in offspring. In this technical commentary, we argue that the method used in the original study inherently amplifies the likelihood of genetic changes that thereafter lead to the heritability of epigenetic changes. We provide evidence that genetic changes from multiple sources do indeed occur in these experiments and explore several avenues by which these changes could be causal to the apparent inheritance of epigenetic changes. We conclude a genetic basis of inheritance cannot be ruled out and thus transgenerational epigenetic inheritance has not been adequately established by the original study.

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来源期刊
Epigenetics
Epigenetics 生物-生化与分子生物学
CiteScore
6.80
自引率
2.70%
发文量
82
审稿时长
3-8 weeks
期刊介绍: Epigenetics publishes peer-reviewed original research and review articles that provide an unprecedented forum where epigenetic mechanisms and their role in diverse biological processes can be revealed, shared, and discussed. Epigenetics research studies heritable changes in gene expression caused by mechanisms others than the modification of the DNA sequence. Epigenetics therefore plays critical roles in a variety of biological systems, diseases, and disciplines. Topics of interest include (but are not limited to): DNA methylation Nucleosome positioning and modification Gene silencing Imprinting Nuclear reprogramming Chromatin remodeling Non-coding RNA Non-histone chromosomal elements Dosage compensation Nuclear organization Epigenetic therapy and diagnostics Nutrition and environmental epigenetics Cancer epigenetics Neuroepigenetics
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