巴拉圭使用的 Sesamum indicum L.品种 "Escoba Blanca "的分子研究

Roberto Martínez-López, W. Pereira, A. Arrúa, Danilo Fernández, L. Centurión
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引用次数: 0

摘要

巴拉圭原产的芝麻质量上乘,因此成为主要出口国之一。在巴拉圭境内的芝麻品种中,分布最广的是 "Escoba Blanca",可能是由于繁殖过程,该品种的等位基因频率、多样性和遗传纯度都发生了变化。开展这项工作的目的是利用微卫星标记确定巴拉圭七家收集 "Escoba Blanca "芝麻的公司的 50 个种群/苗床/库之间的遗传差异。这七个库/公司/合作社收集并代表了来自该国东部和西部地区(查科)不同省份的所有生产者/种子基地的样本,他们与这些生产者/种子基地合作,销售并收集芝麻。从专门为此目的种植的秧苗中获取植物组织提取 DNA,使用的是所有包含的样本/品种。使用了六个微卫星标记:GBssrsa184、GBssrsa123、GBssrsa182、GBssrsa108、GBssrsa08 和 GBssrsa72。对等位基因的数量和频率、距离/分组、种群间的分化及其遗传结构进行了计算。每个位点的平均等位基因数从 1.33 到 3.00 不等。在标记 GBssrsa184 和 GBssrsa108 中,三个种群的等位基因频率较高。受检种群之间的遗传分化程度较高,分别鉴定出4个纯度较高和较低的群体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular study of the “Escoba Blanca” variety of Sesamum indicum L. used in Paraguay
The high quality of sesame seeds originating in the country has led Paraguay to be among the main exporters. Of the varieties available in Paraguayan territory, the most widespread is ‘‘Escoba Blanca’’, which, possibly due to the multiplication process, could promote changes in its allele frequency, diversity, and genetic purity. This work was carried out, aiming to determine the genetic differentiation between 50 populations/seedbeds/banks from seven Paraguayan companies collecting ‘‘Escoba Blanca’’ sesame, using microsatellite markers. These seven banks/companies/cooperatives collect and represent samples from all the producers/seedbeds located in different departments of the Eastern and Western Region (Chaco) of the country, with whom they work, market, and collect sesame. Plant tissue was obtained to extract DNA, from seedlings planted especially for the purpose, using all the included samples/accessions. Six microsatellite markers were used: GBssrsa184, GBssrsa123, GBssrsa182, GBssrsa108, GBssrsa08, and GBssrsa72. The following were calculated: number and frequency of alleles, distance/groupings, differentiation between populations, and their genetic structure. The mean number of alleles per locus ranged from 1.33 to 3.00. In the markers, GBssrsa184 and GBssrsa108, three populations presented a higher frequency of alleles. The populations examined exhibited a wide degree of genetic differentiation between them, with the identification of four groups, with greater and less purity respectively. Keywords: genetic diversity, genotype, microsatellite markers, Sesamum indicum L.
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