用不同精液扩展剂稀释的金丝雀精液对人工授精中卵子的体内影响

A. Özkök
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引用次数: 0

摘要

本研究测定了杜氏磷酸盐缓冲盐水(DPBS)和杜氏改良老鹰培养基(DMEM)扩展剂对人工授精的影响。为此,研究测定了受精卵数、受精卵总数、孵化卵数和受精卵孵化幼体数。根据这些数值计算出受精率(%)、卵孵化率(%)和受精卵孵化率(%)。每组使用 12 只雌雄金丝雀(格罗斯特品种;1-2 岁)。研究共分三组,一组为对照组(C),另一组为两个处理组(T-1、T-2)。C 组的雌性金丝雀不使用任何延长剂,直接进行人工授精。T-1 组雌性金丝雀的精液用 DPBS 以 1:1 稀释;T-2 组雌性金丝雀的精液用 DMEM 以 1:1 稀释。虽然各组在受精率方面存在明显差异(P0.05),但T-1 组的受精率最高(61.81%),T-2 组的受精率最低(61.81%)。繁殖率最高的是 C 组(61.81%),最低的是 T-1 组(29.17%)。(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-Vivo Effects of Canary (Serinus Canary) Semen Diluted with Different Semen Extenders on Eggs in Artificial Insemination
In this study, the effect of Dulbecco's Phosphate Buffered Saline (DPBS) and Dulbecco's Modified Eagle's Medium (DMEM) extenders on artificial insemination was determined.. For this purpose, the number of fertile eggs, the total number of eggs, the number of hatched eggs, and the number of hatchlings from fertile eggs were determined in the study. From these values, the fertility rate (%), egg hatch rate (%) and fertile egg hatch rate (%) were calculated. Twelve male and female canaries (Gloster breed; 1-2 years old) were used in each group. In the study, three groups, one control (C) and the other two treatment groups (T-1, T-2) were formed. Female canaries in group C were directly inseminated without using any extenders. Females in the T-1 group were diluted 1:1 with DPBS; females in the T-2 group were inseminated with semen diluted 1:1 with DMEM. Although there was a significant difference between the groups in terms of fertility rate (P0.05). The highest fertility rate (61.81%) was in group C; the lowest was in the T-1 (29.17%) group. (P
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