{"title":"通过光谱和色谱分析研究添加抗氧化剂的护肤霜对皮肤模拟环境中紫外线-B 诱导的氧化作用的影响","authors":"Temel Kan Bakır, Hüseyin Kastrati","doi":"10.1007/s00769-024-01574-1","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, lipid oxidation kinetics were examined with in vitro methods to investigate whether the antioxidant substances in the final product formulations of four antioxidant-added skin care creams have an antioxidant effect on the skin layers. For the determination of primary and secondary products, iron (III)-thiocyanate (Fe(III)-SCN) and thiobarbituric acid reactive substance methods (TBARS) were used in Cu(II)-induced linoleic acid (LA) emulsions, respectively. It was found that THCl and THC2 coded cream samples slowed down the lipid oxidation rate, while THC3 and THC4 coded samples accelerated the oxidation. The inhibition effects of the same cream samples were confirmed using the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging method. In addition, UV-B-induced oxidation of the LA emulsion containing soy lecithin + Tween 20 emulsifier (HLB:10), prepared as a skin simulation medium for cream samples, was investigated at 35 °C and pH 5.5. The same results were supported by conjugated diene absorbance measurements. Kinetic data were explained by GC–MS analysis. Accordingly, it was observed that the degradation of fatty acids with 17 and 19 carbons into secondary products with low carbon numbers during the development of oxidation occurred at a lower rate in THCl and THC2 coded samples compared to THC3 and THC4 coded samples. The results of this study will raise consumer awareness about these high-cost cosmetic products and enable manufacturers to offer these products to the market with accurate information to users. Conducting in vivo studies supporting this study will provide resources for production suitable for human health.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":454,"journal":{"name":"Accreditation and Quality Assurance","volume":"29 2","pages":"129 - 139"},"PeriodicalIF":0.8000,"publicationDate":"2024-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation of the effect of antioxidant-added skin care creams on UV-B-induced oxidation in skin simulation environment by spectroscopic and chromatographic analyses\",\"authors\":\"Temel Kan Bakır, Hüseyin Kastrati\",\"doi\":\"10.1007/s00769-024-01574-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In this study, lipid oxidation kinetics were examined with in vitro methods to investigate whether the antioxidant substances in the final product formulations of four antioxidant-added skin care creams have an antioxidant effect on the skin layers. For the determination of primary and secondary products, iron (III)-thiocyanate (Fe(III)-SCN) and thiobarbituric acid reactive substance methods (TBARS) were used in Cu(II)-induced linoleic acid (LA) emulsions, respectively. It was found that THCl and THC2 coded cream samples slowed down the lipid oxidation rate, while THC3 and THC4 coded samples accelerated the oxidation. The inhibition effects of the same cream samples were confirmed using the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging method. In addition, UV-B-induced oxidation of the LA emulsion containing soy lecithin + Tween 20 emulsifier (HLB:10), prepared as a skin simulation medium for cream samples, was investigated at 35 °C and pH 5.5. The same results were supported by conjugated diene absorbance measurements. Kinetic data were explained by GC–MS analysis. Accordingly, it was observed that the degradation of fatty acids with 17 and 19 carbons into secondary products with low carbon numbers during the development of oxidation occurred at a lower rate in THCl and THC2 coded samples compared to THC3 and THC4 coded samples. The results of this study will raise consumer awareness about these high-cost cosmetic products and enable manufacturers to offer these products to the market with accurate information to users. Conducting in vivo studies supporting this study will provide resources for production suitable for human health.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":454,\"journal\":{\"name\":\"Accreditation and Quality Assurance\",\"volume\":\"29 2\",\"pages\":\"129 - 139\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2024-02-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accreditation and Quality Assurance\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00769-024-01574-1\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accreditation and Quality Assurance","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s00769-024-01574-1","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Investigation of the effect of antioxidant-added skin care creams on UV-B-induced oxidation in skin simulation environment by spectroscopic and chromatographic analyses
In this study, lipid oxidation kinetics were examined with in vitro methods to investigate whether the antioxidant substances in the final product formulations of four antioxidant-added skin care creams have an antioxidant effect on the skin layers. For the determination of primary and secondary products, iron (III)-thiocyanate (Fe(III)-SCN) and thiobarbituric acid reactive substance methods (TBARS) were used in Cu(II)-induced linoleic acid (LA) emulsions, respectively. It was found that THCl and THC2 coded cream samples slowed down the lipid oxidation rate, while THC3 and THC4 coded samples accelerated the oxidation. The inhibition effects of the same cream samples were confirmed using the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging method. In addition, UV-B-induced oxidation of the LA emulsion containing soy lecithin + Tween 20 emulsifier (HLB:10), prepared as a skin simulation medium for cream samples, was investigated at 35 °C and pH 5.5. The same results were supported by conjugated diene absorbance measurements. Kinetic data were explained by GC–MS analysis. Accordingly, it was observed that the degradation of fatty acids with 17 and 19 carbons into secondary products with low carbon numbers during the development of oxidation occurred at a lower rate in THCl and THC2 coded samples compared to THC3 and THC4 coded samples. The results of this study will raise consumer awareness about these high-cost cosmetic products and enable manufacturers to offer these products to the market with accurate information to users. Conducting in vivo studies supporting this study will provide resources for production suitable for human health.
期刊介绍：
Accreditation and Quality Assurance has established itself as the leading information and discussion forum for all aspects relevant to quality, transparency and reliability of measurement results in chemical and biological sciences. The journal serves the information needs of researchers, practitioners and decision makers dealing with quality assurance and quality management, including the development and application of metrological principles and concepts such as traceability or measurement uncertainty in the following fields: environment, nutrition, consumer protection, geology, metallurgy, pharmacy, forensics, clinical chemistry and laboratory medicine, and microbiology.
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