提取鱼胶原蛋白并确定其特性以增强 A549 细胞株的伤口愈合活性

Vimal Arasan Anbuselvan, Bliss Shiny Nelson, Bhavya Karunaharan, Sivakumar Kandhasamy
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引用次数: 0

摘要

胶原蛋白是一种蛋白质物质,大量存在于动物组织中,能增强组织的物理强度和稳定性。从动物组织中提取的胶原蛋白已被应用于生物医学和保健领域。近年来,水生生物在提取和纯化胶原蛋白方面显示出巨大的潜力。在本研究中,我们选择了三种鱼类:从它们的皮肤和鳞片中提取胶原蛋白。生物质经过预处理,以去除任何非胶原物质和脂肪。使用乙酸提取酸溶性胶原蛋白,最大提取率为 51.55±1.02%。测量了胶原提取物的总蛋白组分和变性温度。使用 SDS-PAGE 分析了胶原蛋白提取物的分子量和亚基组成,发现存在两条 α 链、一条 β 链和一条 γ 链。水解后在氨基酸分析仪中进行了氨基酸分析,结果显示氨基酸总含量中约有三分之一是甘氨酸。在不同的 pH 值和盐度范围内,对胶原蛋白提取物的溶解特性进行了分析。研究了胶原提取物对四种细菌的抗菌活性,结果证明皮肤提取的胶原比鳞片提取物具有更强的抗菌潜力。使用在 F-12K 培养基中培养的 A549 细胞系对提取的胶原蛋白的伤口愈合能力进行了体外研究,结果表明添加胶原蛋白的分散细胞系的细胞再生率达到 95%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extraction and Characterization of Fish Collagen for Enhanced Wound Healing Activity on A549 Cell Lines
Collagen is a proteinaceous material that is present abundantly in animal tissues and gives physical strength and stability to the tissues. Collagen extracted from animal tissues has been applied in the biomedical and healthcare sectors. In recent days, aquatic organisms have shown great potential for collagen extraction and purification. In the present study, we selected three fish species: Naucrates ductor, Oreochromis mossambicus and Cyprinus carpiofor collagen extraction from both their skin and scales. The biomass was pre-treated to remove any non-collagenous matter and fat. Acid-soluble collagen was extracted using acetic acid and a maximum extraction of 51.55±1.02% was reported. The total protein fractions and the denaturation temperatures of the collagen extractswere measured. The molecular weights and the subunit compositions of the collagen extracts were analyzed using SDS-PAGE and the presence of two α chains, one β, and one γ chain. Amino acid profiling was done in an amino acid analyzer after hydrolysis and about one third of the total amino acid content was glycine. The solubility characteristics of the collagenextracts were analyzed at different ranges of pH and salinity. The antimicrobial activities of the collagen extracts were examined against four bacterial species, and it was proven that skin-derived collagens had greater antibacterial potential than extracts of the scales. In vitro studies on the wound healing capacity of the extracted collagen were performed using A549 cell line cultured in F-12K medium, and 95% of cell regeneration was observed in collagen-supplemented dispersed cell lines.  
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