酸性丙酸丙酸杆菌 CDBB-B-1981 从经蒸汽爆炸预处理的龙舌兰甘蔗渣酶水解物中生产丙酸的情况

Veronica Duran‐Cruz, Sergio Hernández, I. Ortíz
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摘要

丙酸(PA)生产的生化途径是一种有趣的替代方法,其中包括利用生物质作为原料。本研究评估了利用龙舌兰甘蔗渣(AB)这种木质纤维素残渣,通过酸性丙酸丙酸丙酸杆菌(Propionibacterium acidipropinici)在批处理系统(125 mL-保温瓶和 1000 mL-生物反应器)中生产 PA 的情况。该工艺包括 142°C 下 15 分钟的汽爆预处理和酶水解,其中对固体负荷(预处理中为 2.75% 和 5%,酶水解中为 2.5%、3.75% 和 5%)进行了评估。此外,还测试了 Cellic® CTec2 的酶浓度为 18.3 滤纸单位(FPU)/gAB(1×)以及 1.5× 和 3×。在预处理中测试的两种固体负荷下获得的总碳水化合物(TC)产量在统计上相似,但 3 倍酶浓度提高了 TC、葡萄糖和木糖的产量(分别为 0.23 ± 0.01、0.15 ± 0.01 和 0.03 ± 0.01 g/gAB)。在这些条件下获得的水解物被评估为生产 PA 的碳源,生产率为 0.069 ± 0.006 g/L h,产品/底物产量为 0.44 gPA/gTC。与不控制 pH 值的密封瓶相比,控制培养物中的 pH 值可将生物反应器中的发酵时间缩短 52%。水解物作为生产 PA 的碳源的潜力得到了证明,因为大约 50% 的初始碳被转化为该产品。所观察到的产品/底物产量与所报道的不同生物质类型、预处理或混合酶水解物以及相同或相关微生物的产量相似。不过,与其他预处理方法相比,所研究的系统具有一些优势,例如不需要添加化学或解毒阶段,温度和时间也更低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Propionic acid production by Propionibacterium acidipropionici CDBB‐B‐1981 from enzymatic hydrolysates of Agave bagasse pretreated by steam explosion
The biochemical pathway for propionic acid (PA) production is an interesting alternative that can include the utilization of biomass as feedstock. This study evaluated the utilization of Agave bagasse (AB), a lignocellulosic residue, to produce PA by Propionibacterium acidipropinici in batch systems (125 mL‐hermetic bottles and 1000 mL‐bioreactor). The process included a steam explosion pretreatment at 142°C for 15 min and enzymatic hydrolysis, where solid loading (2.75% and 5% in pretreatment and 2.5%, 3.75%, and 5% in enzymatic hydrolysis) was evaluated. Furthermore, the enzymatic concentrations of 18.3 filter paper unit (FPU)/gAB (1×) of Cellic® CTec2 and 1.5× and 3× were tested. The yields of total carbohydrates (TC) obtained at the two solid loadings tested in the pretreatment were statistically similar, but the 3x enzymatic concentration enhanced the yields of TC, glucose, and xylose (0.23 ± 0.01, 0.15 ± 0.01 and 0.03 ± 0.01 g/gAB, respectively). The hydrolysates obtained under these conditions were evaluated as carbon sources for PA production, obtaining a productivity of 0.069 ± 0.006 g/L h and a yield product/substrate of 0.44 gPA/gTC. The control of pH in the culture reduced the fermentation time in the bioreactor by 52% compared with the hermetic bottles without pH control. The potential of hydrolysates as carbon sources for PA production was evidenced, as approximately 50% of the initial carbon was converted to this product. The observed yield product/substrate was similar to those reported from hydrolysates of diverse biomass types, pretreatments, or enzymatic cocktails and the same or related microorganisms. However, the system studied has advantages, such as not requiring the addition of chemical or detoxification stage, and lower temperature and time compared to other pretreatments.
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