日粮对羊绒山羊瘤胃产甲烷古细菌组成和多样性的影响

IF 0.7 4区 农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
K. N. Li, Y. R. Wei, R. H. Na
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引用次数: 0

摘要

日粮是影响瘤胃微生物群落的最直接方式。本研究旨在探讨不同日粮对羊绒山羊瘤胃甲烷菌组成的影响。24 只羊绒山羊被随机分为四组,分别饲喂苜蓿干草 (AH)、苜蓿干草加精料 (AHC)、玉米秆 (CS) 和玉米秆加精料 (CSC)。饲喂 AHC 组的氨氮浓度大大高于其他组。AH组和AHC组的总挥发性脂肪酸、乙酸盐和丙酸盐浓度高于CS组和CSC组。根据高通量测序方法,不同组别的古细菌丰度存在明显差异。在 AH 组中,嗜甲烷杆菌的丰度为 21.28%,高于其他组。在 AH 组和 AHC 组中,未分类甲烷菌是主要的甲烷菌,其丰度分别为 48.09% 和 44.97%;在 CS 组和 CSC 组中,甲烷杆菌是主要的甲烷菌,其丰度分别为 41.22% 和 20.73%。pH 值与甲烷磷菌之间呈正相关;醋酸盐与未分类甲烷菌之间呈负相关。总之,不同日粮中羊绒山羊的甲烷菌群落各不相同。在以苜蓿干草为基础的日粮中,隶属于Methanomassiliicoccales的甲烷菌群是山羊瘤胃中的主要甲烷菌。然而,当饲喂以玉米秸秆为基础的日粮时,瘤胃液中的主要甲烷菌是甲烷杆菌。这些结果与日粮成分有关,尤其是粗蛋白含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of diet on ruminal methanogenic archaea composition and diversity in cashmere goats
Diet is the most direct way to affect the rumen microbial community. The objective of this study was to examine the effects of different diets on ruminal methanogen composition in cashmere goats. Twenty four cashmere goats were randomly divided into four groups that were fed alfalfa hay (AH), alfalfa hay plus concentrate (AHC), corn stalks (CS), and corn stalks plus concentrate (CSC). The concentration of ammonia N for the group fed AHC was substantially higher than in the other groups. The concentrations of total VFAs, acetate, and propionate in AH and AHC groups were higher than in the CS and CSC groups. The abundance of archaea was markedly different among different groups based on a high throughput sequencing method. The abundance of Candidatus_Methanomethylophilus was 21.28% in the AH group, which was higher than the other groups. Unclassified_c_Thermoplasmata was the dominant methanogen in the AH and AHC groups, with abundances of 48.09% and 44.97%, respectively; Methanobrevibacter was the dominant methanogen in the CS and CSC groups, with abundances of 41.22% and 20.73%, respectively. A positive correlation was identified between pH and Methanosphaera; a negative relationship was observed between acetate and Unclassified_o_Thermoplasmatales. In conclusion, the methanogen communities in cashmere goats varied on different diets. In the alfalfa hay based diets, the Methanomassiliicoccales affiliated groups were the dominant methanogens in the rumen of the goats. However, when fed the corn stalk based diets, Methanobrevibacter was the dominant methanogen in the rumen fluid. The results were related to dietary composition, especially crude protein content.
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来源期刊
South African Journal of Animal Science
South African Journal of Animal Science 农林科学-奶制品与动物科学
CiteScore
1.50
自引率
0.00%
发文量
39
审稿时长
>36 weeks
期刊介绍: The South African Journal of Animal Science is an open access, peer-reviewed journal for publication of original scientific articles and reviews in the field of animal science. The journal publishes reports of research dealing with production of farmed animal species (cattle, sheep, goats, pigs, horses, poultry and ostriches), as well as pertinent aspects of research on aquatic and wildlife species. Disciplines covered nutrition, genetics, physiology, and production systems. Systematic research on animal products, behaviour, and welfare are also invited. Rigorous testing of well-specified hypotheses is expected.
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