通过电刺激促进间充质干细胞在聚己内酯/MoS2 纳米纤维支架上的成骨分化

IF 3.2 3区 工程技术 Q2 CHEMISTRY, PHYSICAL
Elahe Amiri, Mehrdad Khakbiz, Behnaz Bakhshandeh, Nika Ranjbar and Javad Mohammadnejad
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引用次数: 0

摘要

骨组织工程(BTE)的最新进展为治疗大面积骨缺损和非愈合性骨缺损提供了替代疗法。最近的研究表明,电刺激(ES)可影响骨细胞并促进骨细胞的粘附、迁移、增殖和分化等过程,从而促进骨再生过程。在这项研究中,我们合成了二硫化钼(MoS2)纳米颗粒(NPs),并将其加入到聚己内酯(PCL)聚合物基质中,以增强支架的导电性。利用扫描电子显微镜(SEM)、水接触角测量、电导率和拉伸强度评估对 PCL/MoS2 纳米复合材料进行了分析。体外研究使用 MTT 试验评估了间充质干细胞(MSCs)的粘附性和所制支架的生物相容性。通过体外模拟体液(SBF)生物矿化测定了生物矿物质晶体沉积,茜素红 S 分析表明 PCL/MoS2 复合支架上的磷酸钙沉积增强。此外,qPCR 分析表明,将 PCL/MoS2 上培养的间充质干细胞暴露于 ES 中两周后,细胞中的成骨标志物(骨钙素(OC)和碱性磷酸酶(ALP))转录上调。单独使用 ES 或分化培养基都能促进成骨。然而,当两种刺激同时使用时,成骨标志物的水平会有所提高。我们的研究结果表明,ES 在促进成骨分化方面起着重要作用,尤其是与作为成骨增强剂的 MoS2NPs 结合使用时。因此,PCL/MoS2 纳米纤维支架可作为 BTE 的合适候选材料,而 ES 作为常用生物材料支架的一种有效工具具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Enhancing osteogenic differentiation of mesenchymal stem cells seeded on a polycaprolactone/MoS2 nanofibrous scaffold through electrical stimulation

Enhancing osteogenic differentiation of mesenchymal stem cells seeded on a polycaprolactone/MoS2 nanofibrous scaffold through electrical stimulation

Recent progress in bone tissue engineering (BTE) has introduced alternative treatments for sizable and non-healing bone defects. Electrical stimulation (ES) has recently been shown to influence bone cells and foster processes such as adhesion, migration, proliferation, and differentiation, which can enhance the bone regeneration process. In this study, we synthesized molybdenum disulfide (MoS2) nanoparticles (NPs) and incorporated them into a polycaprolactone (PCL) polymeric matrix to enhance the electrical conductivity of scaffolds. The PCL/MoS2 nanocomposites were analysed using scanning electron microscopy (SEM), water contact angle measurement, electrical conductivity, and tensile strength assessments. In vitro studies evaluated the adhesion of mesenchymal stem cells (MSCs) and the biocompatibility of the fabricated scaffolds using the MTT assay. Biomineral crystal deposition was determined via in vitro simulated body fluid (SBF) biomineralization, and alizarin red S assays demonstrated enhanced calcium phosphate deposition on the PCL/MoS2 composite scaffold. Additionally, qPCR analysis revealed that exposing MSCs cultured on PCL/MoS2 to ES for two weeks transcriptionally upregulated osteogenic markers (osteocalcin (OC) and alkaline phosphatase (ALP)) in cells. Using either ES or a differentiation medium alone could enhance osteogenesis. However, when both stimuli were applied concurrently, improved levels of osteogenic markers were observed. Our findings suggest that ES plays a significant role in boosting osteogenic differentiation, particularly when combined with MoS2NPs as an osteogenic enhancer. Therefore, PCL/MoS2 nanofibrous scaffolds can be proposed as suitable candidates for BTE, and ES holds great potential as an effective tool along with commonly used biomaterial scaffolds.

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来源期刊
Molecular Systems Design & Engineering
Molecular Systems Design & Engineering Engineering-Biomedical Engineering
CiteScore
6.40
自引率
2.80%
发文量
144
期刊介绍: Molecular Systems Design & Engineering provides a hub for cutting-edge research into how understanding of molecular properties, behaviour and interactions can be used to design and assemble better materials, systems, and processes to achieve specific functions. These may have applications of technological significance and help address global challenges.
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