{"title":"生物多样性研究中的样本设计很重要：对劳伦斯绒虫 Peripatopsis lawrencei（Onychophora: Peripatopsidae）的精细研究揭示了隐藏的多样性","authors":"Julian A. Nieto Lawrence, Savel R. Daniels","doi":"10.1071/is23051","DOIUrl":null,"url":null,"abstract":"<p>A fine-scale phylogenetic and phylogeographic analysis of <i>Peripatopsis lawrencei</i> s.l. was conducted with both mitochondrial and nuclear DNA sequence data, using both external morphology and scanning electron microscopy of taxonomically important characters. A total of 119 sequences were used for the mitochondrial cytochrome <i>c</i> oxidase subunit I (<i>COI</i>) whereas a single representative specimen from each locality was sequenced for the nuclear <i>18S</i> rRNA locus. Phylogenetic analyses were conducted on the total <i>COI</i> data set and the combined <i>COI</i> + <i>18S</i> rRNA data set using a Bayesian analysis and maximum likelihood analyses. For the combined DNA sequence data set, a divergence time estimation was further undertaken in BEAST and specimens placed in a phylogenetic framework including all the described <i>Peripatopsis</i> species from South Africa. In addition, a phylogeographic study was conducted exclusively on <i>P. lawrencei</i> s.s. (clade A) using an analysis of molecular variance and haplotype network. Phylogenetic results indicated that, at the Oubos sample locality, two highly distinct genetic lineages were present (clades A and B), whereas a divergence time estimation suggests a Miocene cladogenesis of the novel Oubos lineage. Marked phylogeographic structure was observed for <i>P. lawrencei</i> s.s. (restricted to clade A) across the distribution range with limited maternal dispersal. Morphologically, the two sympatric lineages at Oubos A and B differed in leg pair number, ventral colour and dorsal scale rank counts, as evident from scanning electron microscopy. Our results support the recognition of a distinct species that occurs in sympatry with <i>P. lawrencei</i> s.s. The new species<i>, P. aereus</i> sp. nov. (clade B) is described and the implication for fine-scale taxonomic studies on saproxylic taxa is discussed.</p><p>ZooBank: urn:lsid:zoobank.org:pub:AB6E0BDA-7B5F-4FD3-A863-BA7C814E278C</p>","PeriodicalId":54927,"journal":{"name":"Invertebrate Systematics","volume":"276 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sample design in biodiversity studies matters: a fine-scale study of Lawrence’s velvet worm, Peripatopsis lawrencei (Onychophora: Peripatopsidae), reveals hidden diversity\",\"authors\":\"Julian A. Nieto Lawrence, Savel R. Daniels\",\"doi\":\"10.1071/is23051\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>A fine-scale phylogenetic and phylogeographic analysis of <i>Peripatopsis lawrencei</i> s.l. was conducted with both mitochondrial and nuclear DNA sequence data, using both external morphology and scanning electron microscopy of taxonomically important characters. A total of 119 sequences were used for the mitochondrial cytochrome <i>c</i> oxidase subunit I (<i>COI</i>) whereas a single representative specimen from each locality was sequenced for the nuclear <i>18S</i> rRNA locus. Phylogenetic analyses were conducted on the total <i>COI</i> data set and the combined <i>COI</i> + <i>18S</i> rRNA data set using a Bayesian analysis and maximum likelihood analyses. For the combined DNA sequence data set, a divergence time estimation was further undertaken in BEAST and specimens placed in a phylogenetic framework including all the described <i>Peripatopsis</i> species from South Africa. In addition, a phylogeographic study was conducted exclusively on <i>P. lawrencei</i> s.s. (clade A) using an analysis of molecular variance and haplotype network. Phylogenetic results indicated that, at the Oubos sample locality, two highly distinct genetic lineages were present (clades A and B), whereas a divergence time estimation suggests a Miocene cladogenesis of the novel Oubos lineage. Marked phylogeographic structure was observed for <i>P. lawrencei</i> s.s. (restricted to clade A) across the distribution range with limited maternal dispersal. Morphologically, the two sympatric lineages at Oubos A and B differed in leg pair number, ventral colour and dorsal scale rank counts, as evident from scanning electron microscopy. Our results support the recognition of a distinct species that occurs in sympatry with <i>P. lawrencei</i> s.s. The new species<i>, P. aereus</i> sp. nov. (clade B) is described and the implication for fine-scale taxonomic studies on saproxylic taxa is discussed.</p><p>ZooBank: urn:lsid:zoobank.org:pub:AB6E0BDA-7B5F-4FD3-A863-BA7C814E278C</p>\",\"PeriodicalId\":54927,\"journal\":{\"name\":\"Invertebrate Systematics\",\"volume\":\"276 1\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-02-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Invertebrate Systematics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1071/is23051\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"EVOLUTIONARY BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Invertebrate Systematics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1071/is23051","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"EVOLUTIONARY BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
利用线粒体和核DNA序列数据，并通过外部形态学和扫描电子显微镜观察分类学上的重要特征，对Peripatopsis lawrencei s.l.进行了精细的系统发育和系统地理学分析。线粒体细胞色素 c 氧化酶亚单位 I（COI）共使用了 119 个序列，而核 18S rRNA 位点则对每个地点的一个代表性标本进行了测序。采用贝叶斯分析法和最大似然法对 COI 数据集和 COI + 18S rRNA 数据集进行了系统发育分析。对于合并的 DNA 序列数据集，进一步在 BEAST 中进行了分歧时间估计，并将标本置于一个系统发育框架中，其中包括南非所有已描述的 Peripatopsis 物种。此外，利用分子方差分析和单体型网络，专门对 P. lawrencei s.s.（支系 A）进行了系统地理学研究。系统发育结果表明，在 Oubos 样地，存在两个高度不同的遗传系（支系 A 和 B），而对分歧时间的估计表明，新的 Oubos 系是中新世的支系形成。在整个分布区观察到明显的系统地理结构，P. lawrencei s.s.（局限于支系 A）的母系扩散有限。从形态上看，扫描电子显微镜显示，奥博斯 A 支系和 B 支系在腿对数、腹面颜色和背鳞等级计数方面存在差异。描述了新种 P. aereus sp. nov.（B 支系），并讨论了对无鞘类群进行精细分类研究的意义。ZooBank: urn:lsid:zoobank.org:pub:AB6E0BDA-7B5F-4FD3-A863-BA7C814E278C
Sample design in biodiversity studies matters: a fine-scale study of Lawrence’s velvet worm, Peripatopsis lawrencei (Onychophora: Peripatopsidae), reveals hidden diversity
A fine-scale phylogenetic and phylogeographic analysis of Peripatopsis lawrencei s.l. was conducted with both mitochondrial and nuclear DNA sequence data, using both external morphology and scanning electron microscopy of taxonomically important characters. A total of 119 sequences were used for the mitochondrial cytochrome c oxidase subunit I (COI) whereas a single representative specimen from each locality was sequenced for the nuclear 18S rRNA locus. Phylogenetic analyses were conducted on the total COI data set and the combined COI + 18S rRNA data set using a Bayesian analysis and maximum likelihood analyses. For the combined DNA sequence data set, a divergence time estimation was further undertaken in BEAST and specimens placed in a phylogenetic framework including all the described Peripatopsis species from South Africa. In addition, a phylogeographic study was conducted exclusively on P. lawrencei s.s. (clade A) using an analysis of molecular variance and haplotype network. Phylogenetic results indicated that, at the Oubos sample locality, two highly distinct genetic lineages were present (clades A and B), whereas a divergence time estimation suggests a Miocene cladogenesis of the novel Oubos lineage. Marked phylogeographic structure was observed for P. lawrencei s.s. (restricted to clade A) across the distribution range with limited maternal dispersal. Morphologically, the two sympatric lineages at Oubos A and B differed in leg pair number, ventral colour and dorsal scale rank counts, as evident from scanning electron microscopy. Our results support the recognition of a distinct species that occurs in sympatry with P. lawrencei s.s. The new species, P. aereus sp. nov. (clade B) is described and the implication for fine-scale taxonomic studies on saproxylic taxa is discussed.
期刊介绍：
Invertebrate Systematics (formerly known as Invertebrate Taxonomy) is an international journal publishing original and significant contributions on the systematics, phylogeny and biogeography of all invertebrate taxa. Articles in the journal provide comprehensive treatments of clearly defined taxonomic groups, often emphasising their biodiversity patterns and/or biological aspects. The journal also includes contributions on the systematics of selected species that are of particular conservation, economic, medical or veterinary importance.
Invertebrate Systematics is a vital resource globally for scientists, students, conservation biologists, environmental consultants and government policy advisors who are interested in terrestrial, freshwater and marine systems.
Invertebrate Systematics is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
