利用从巨聚体 PCR 构建串联重复序列中提取的标准，确定转基因小鼠中改良 Cre 的基因组拷贝数

Q1 Biochemistry, Genetics and Molecular Biology

Frontiers in Life Science Pub Date : 2024-02-12 DOI:10.1080/26895293.2024.2316078

Ge Diao, Min Tian, Runbo Li, Jie Huang, Jian Han, Jianxin Guo

引用次数: 0

摘要

确定 Cre 转基因的基因组拷贝数对于建立转基因动物模型至关重要。我们展示了一种实时定量聚合酶链反应（RT-qPCR） ...

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The genomic copy number determination of improved Cre in transgenic mice using standards derived from megaprimer PCR constructed tandem repeats

Determination of the genomic copy number of Cre transgene is critical for the establishment of transgenic animal models. We demonstrate a real-time quantitative polymerase chain reaction (RT-qPCR) ...

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来源期刊

Frontiers in Life Science MULTIDISCIPLINARY SCIENCES-

CiteScore

5.50

自引率

0.00%

发文量

期刊介绍： Frontiers in Life Science publishes high quality and innovative research at the frontier of biology with an emphasis on interdisciplinary research. We particularly encourage manuscripts that lie at the interface of the life sciences and either the more quantitative sciences (including chemistry, physics, mathematics, and informatics) or the social sciences (philosophy, anthropology, sociology and epistemology). We believe that these various disciplines can all contribute to biological research and provide original insights to the most recurrent questions.