J M Rasmussen, H V Marquart, R Rask, H H Jepsen, S E Svehag
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引用次数: 6
摘要
采用间接免疫酶法测定未标记单克隆抗CR2抗体(OKB7和HB-5)的结合,定量测定C3dg/ ep斯坦-巴尔病毒受体(CR2)在人外周血、扁桃体B淋巴细胞和Raji细胞上的表达。饱和结合曲线的Scatchard分析显示,Raji细胞对OKB7和HB-5平均表达约22,000和17,000个结合位点,平均亲和力常数分别为9.9 X 10(10)和8.7 X 10(10) M-1。扁桃体单个核细胞(TMC)对OKB7和HB-5分别表达16700个和17600个结合位点,对HB-5的亲和力常数(3.2 X 10(10) M-1)明显低于OKB7 (9.0 X 10(10) M-1)。来自健康供体的平均34%的E外周血单个核细胞(PBMC)和49%的TMC表达CR2抗原。当校正CR2阳性细胞的比例时,E- PBMC的平均CR2密度为12,600 (n = 10), TMC的平均CR2密度为34,000 (n = 4)。
Quantification of C3dg/Epstein-Barr virus receptors on human B cells and B cell lines.
The expression of C3dg/Epstein-Barr virus receptors (CR2) on human peripheral blood and tonsillar B lymphocytes and Raji cells was quantified by measuring binding of unlabeled monoclonal anti-CR2 antibody (OKB7 and HB-5) in an indirect immunoenzymatic assay. Scatchard analysis of saturation binding curves revealed that Raji cells on the average express about 22,000 and 17,000 binding sites, with mean affinity constants of 9.9 X 10(10) and 8.7 X 10(10) M-1 for OKB7 and HB-5, respectively. Tonsillar mononuclear cells (TMC) expressed 16,700 and 17,600 binding sites for OKB7 and HB-5, respectively, with a significantly lower affinity constant for HB-5 (3.2 X 10(10) M-1) than for OKB7 (9.0 X 10(10) M-1). On the average 34% of E- peripheral blood mononuclear cells (PBMC) from healthy donors and 49% of TMC expressed the CR2 antigen. When correcting for the fraction of CR2-positive cells, the mean CR2 density was 12,600 on E- PBMC (n = 10) and 34,000 on TMC (n = 4).