地塞米松和替诺昔康对不同铜绿假单胞菌临床分离株毒力活性的影响

IF 1.7 Q3 INFECTIOUS DISEASES
GERMS Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI:10.18683/germs.2023.1401
Heba E AboElfarh, El-Sayed E Habib, Mohamed M A El-Sokkary
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引用次数: 0

摘要

引言本研究旨在探讨常用非抗生素药物(地塞米松和替诺昔康)对铜绿假单胞菌感染治疗、抗生素耐药性和该病原体毒力的影响:研究了四种抗生素(庆大霉素、头孢吡肟、环丙沙星和美罗培南)。蛋白质溶解和溶血被选为研究的毒力因子。在这项工作中,我们选择了以下最终浓度:地塞米松(0.0052 μg/mL)和替诺昔康(2.7 μg/mL)与抗生素联合使用或单独使用,以研究它们对铜绿假单胞菌分离株的抗生素耐药性和毒力的影响:结果:这些药物仅在 0-3 个分离株中增加或减少了抗生素耐药性,这表明所研究的药物对抗生素耐药性没有显著影响。有趣的是,我们的研究表明地塞米松和替诺昔康都增加了所调查分离物的溶血活性。另一方面,我们的研究结果表明,地塞米松对蛋白水解活性没有总体的最终增减效应,而替诺昔康则会增加所研究分离物的蛋白水解活性。有趣的是,通过实时聚合酶链式反应(real-time PCR),地塞米松明显下调了毒力基因(即 algD、plcH 和 toxA)。然而,在另一个分离物中观察到了负面影响。遗憾的是,在替诺昔康与庆大霉素联用的情况下,只在一个分离物中观察到了积极影响:结论:铜绿假单胞菌对庆大霉素和环丙沙星的耐药性可能会受到这些抗生素与地塞米松或替诺昔康联合使用的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of dexamethasone and tenoxicam on the virulence activities of different Pseudomonas aeruginosa clinical isolates.

Introduction: This study aimed to examine the effect of commonly used non-antibiotic drugs (dexamethasone and tenoxicam), on treatment of Pseudomonas aeruginosa infections, antibiotic resistance and virulence in this pathogen.

Methods: Four antibiotics (gentamicin, cefepime, ciprofloxacin and meropenem) were investigated. The proteolysis and hemolysis were selected as virulence factors for investigation. In this work, we selected the following final concentrations: dexamethasone (0.0052 μg/mL) and tenoxicam (2.7 μg/mL) to be used in combination with antibiotics or alone for investigation of their effects on antibiotic resistance and virulence in P. aeruginosa isolates.

Results: The drugs either increased or decreased antibiotic resistance in only 0-3 isolates, which indicates that the investigated drugs did not significantly affect the antibiotic resistance. Interestingly, our study demonstrated that both dexamethasone and tenoxicam increased the hemolytic activity of the investigated isolates. On the other hand, our results indicated that no overall final increasing or decreasing effect could be observed for dexamethasone on the proteolytic activity, while tenoxicam increased the proteolytic activity of the investigated isolates. Interestingly, by real-time PCR dexamethasone has shown significant down-regulation of virulence genes namely algD, plcH and toxA, apparently, in case of combination with ciprofloxacin and with gentamicin in one isolate. However, a negative influence was observed in another isolate. Unfortunately, in the case of tenoxicam the only positive effect was observed in the combination with gentamicin in one isolate.

Conclusions: Resistance of P. aeruginosa against gentamicin and ciprofloxacin may be affected by combining these antibiotics with dexamethasone or tenoxicam.

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来源期刊
GERMS
GERMS INFECTIOUS DISEASES-
CiteScore
2.80
自引率
5.00%
发文量
36
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