使用基于 ImageJ 的脚本，通过荧光显微镜检测复制应激和相关的细胞周期从 G2 期退出。

4区生物学 Q4 Biochemistry, Genetics and Molecular Biology

Methods in cell biology Pub Date : 2024-01-01 Epub Date: 2023-03-25 DOI:10.1016/bs.mcb.2023.02.003

Arne Lindqvist, Zhiyu Hao, Karen Akopyan

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引用次数: 0

摘要

复制压力会危及基因组的完整性。根据程度的不同，复制应激会导致细胞在 S 期的进展减慢，进入 G2 期时出现 DNA 损伤。在 G2 期，细胞要么恢复并最终进入有丝分裂，要么永久退出细胞周期。在此，我们介绍一种利用荧光显微镜检测细胞周期分布、复制应激和细胞周期退出 G2 期的方法。我们提供了一个使用 ImageJ 自动分析的脚本。我们的重点是让没有丰富计算机知识的人也能使用脚本和设置。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy.

Replication stress risks genomic integrity. Depending on the level, replication stress can lead to slower progression through S phase and entry into G2 phase with DNA damage. In G2 phase, cells either recover and eventually enter mitosis or permanently withdraw from the cell cycle. Here we describe a method to detect cell cycle distribution, replication stress and cell cycle exit from G2 phase using fluorescence microscopy. We provide a script to automate the analysis using ImageJ. The focus has been to make a script and setup that is accessible to people without extensive computer knowledge.

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来源期刊

Methods in cell biology 生物-细胞生物学

CiteScore

3.10

自引率

0.00%

发文量

125

审稿时长

3 months

期刊介绍： For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.