对鬣羚(Lama guanicoe)怀孕期间荷尔蒙波动的无创评估及其在野生种群中的应用。

IF 2.6 3区 环境科学与生态学 Q2 BIODIVERSITY CONSERVATION
Conservation Physiology Pub Date : 2024-02-09 eCollection Date: 2024-01-01 DOI:10.1093/conphys/coae003
A Marozzi, V I Cantarelli, A Panebianco, F M Gomez, R Ovejero, P F Gregorio, F Peña, M F Ponzio, P D Carmanchahi
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引用次数: 0

摘要

利用非侵入性方法测量粪便中的激素代谢物来获取内分泌概况,是监测野生物种卵巢活动和怀孕情况的一种广泛应用的方法。这些工具可以在不对个体造成捕获相关压力的情况下获取生理信息。在这项研究中,我们的目标是:1)从生物学角度验证一种非侵入性方法,以评估鬣羚(Lama guanicoe)妊娠期间粪便中孕激素和雌激素的波动;2)将这种技术应用于评估野生放养种群的妊娠情况。在一个 6.5 公顷围场中收集了雌性瓜纳卡鼠妊娠期(约 12 个月)的粪便样本。结果发现两种激素的粪便代谢物都有所增加。孕激素逐渐增加,而雌激素在妊娠期的大部分时间都保持在基础值,仅在产仔前几天达到峰值。为了评估野生放养动物的妊娠情况,我们在拉帕尤尼亚省级保护区(阿根廷门多萨)的一个种群中收集了妊娠初期和妊娠末期的粪便样本。在可能怀孕的前几个月,怀孕的雌性占种群的 40% 到 80%;在妊娠末期,只有 20% 到 40% 的雌性确认怀孕。我们的研究结果表明,这里使用的多克隆抗血清和性激素代谢物检测方法可以检测到鬣羚通过粪便排泄的代谢物的变化,为对雌性生殖状况进行非侵入性激素监测提供了可能。此外,野生条件下的研究结果表明,自然流产可能发生在妊娠期的头几个月。虽然有些流产可能是自然的,但恶劣的环境条件对如此漫长的妊娠过程的支持提出了挑战,这可能是另一个需要考虑的相关因素。本文的研究结果加深了我们对南美洲最具代表性的有蹄类动物之一的生殖生理的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Non-invasive assessment of hormonal fluctuations during pregnancy in guanacos (Lama guanicoe) and its application in a wild population.

Obtaining endocrinological profiles using non-invasive methodologies by the measurement of hormone fecal metabolites is a widely used method to monitor ovarian activity and pregnancy in wild species. These tools allow the obtention of physiological information without causing capture-related stress on the individuals. In this research, we aimed to 1) biologically validate a non-invasive method to assess fecal progestagens and estrogens fluctuations during gestation in guanacos (Lama guanicoe) and 2) apply this technique to assess pregnancy in a wild free-ranging population. Fecal samples were collected through the gestation period (~12 months) of female guanacos in a 6.5-ha paddock. An increase in fecal metabolites of both hormones was detected. Progestagens increased gradually, in contrast to estrogens, which remained at basal values for most of the gestation period and peaked only a few days before calving. To assess pregnancy in wild free-ranging animals, fecal samples were collected from a population of La Payunia provincial reserve (Mendoza, Argentina) during the beginning of gestation and at the end of gestation. Through the first months of possible gestation, pregnant females represented between 40 and 80% of the population; at the end of gestation, only 20-40% of the females had confirmed pregnancies. Our results demonstrated that the polyclonal antisera and sexual hormone metabolite assays used here detect variations in the metabolites excreted through feces in guanacos and provide the possibility of non-invasive hormone monitoring of female reproductive status. Also, the findings in wild conditions suggest that natural abortions could have occurred during the first months of gestation. Although some abortions may be natural, the harsh environmental conditions that challenge the support of such a long gestational process may be another relevant factor to consider. The results obtained here enhance our understanding of the reproductive physiology of one of the most emblematic ungulates in South America.

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来源期刊
Conservation Physiology
Conservation Physiology Environmental Science-Management, Monitoring, Policy and Law
CiteScore
5.10
自引率
3.70%
发文量
71
审稿时长
11 weeks
期刊介绍: Conservation Physiology is an online only, fully open access journal published on behalf of the Society for Experimental Biology. Biodiversity across the globe faces a growing number of threats associated with human activities. Conservation Physiology will publish research on all taxa (microbes, plants and animals) focused on understanding and predicting how organisms, populations, ecosystems and natural resources respond to environmental change and stressors. Physiology is considered in the broadest possible terms to include functional and mechanistic responses at all scales. We also welcome research towards developing and refining strategies to rebuild populations, restore ecosystems, inform conservation policy, and manage living resources. We define conservation physiology broadly and encourage potential authors to contact the editorial team if they have any questions regarding the remit of the journal.
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