比较基因组学表明,SAD基因簇的串联重复是导致S. hispanica种子中α-亚麻酸(ω-3)含量增加的驱动因素。

IF 3.9 2区 生物学 Q1 GENETICS & HEREDITY
Plant Genome Pub Date : 2024-03-01 Epub Date: 2024-02-10 DOI:10.1002/tpg2.20430
Tannaz Zare, Jeff F Paril, Emma M Barnett, Parwinder Kaur, Rudi Appels, Berit Ebert, Ute Roessner, Alexandre Fournier-Level
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引用次数: 0

摘要

莎草(Salvia hispanica L.)含有丰富的ω-3 多不饱和脂肪酸(ω-3-PUFAs),对人体健康非常有益。通过组装和比较分析西班牙糙米的染色体级参考基因组,研究了这种新兴作物中ω-3-PUFA含量增加的基因组基础。高度连续的 321.5-Mbp 基因组组装覆盖了所有六条染色体,从而鉴定出 32,922 个编码蛋白质的基因。在 S. hispanica 系中发现了两个全基因组重复(WGD)事件。然而,根据系统进化组学,这些 WGD 事件无法与 S. hispanica 种子中的α-亚麻酸(ALA,ω-3)高积累联系起来。相反,我们的分析支持这样的假设,即通过特定脂质基因家族(尤其是硬脂酰-酰基载体蛋白去饱和酶(ShSAD)基因家族)的串联重复而实现的进化扩张,是茄子种子中ω-3-PUFAs丰度的主要驱动力。从 S. hispanica 基因组分析中获得的见解将有助于确立分子育种目标,通过基因组编辑技术提高油料作物中的ω-3 含量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative genomics points to tandem duplications of SAD gene clusters as drivers of increased α-linolenic (ω-3) content in S. hispanica seeds.

Salvia hispanica L. (chia) is a source of abundant ω-3 polyunsaturated fatty acids (ω-3-PUFAs) that are highly beneficial to human health. The genomic basis for this accrued ω-3-PUFA content in this emerging crop was investigated through the assembly and comparative analysis of a chromosome-level reference genome for S. hispanica. The highly contiguous 321.5-Mbp genome assembly covering all six chromosomes enabled the identification of 32,922 protein-coding genes. Two whole-genome duplications (WGD) events were identified in the S. hispanica lineage. However, these WGD events could not be linked to the high α-linolenic acid (ALA, ω-3) accumulation in S. hispanica seeds based on phylogenomics. Instead, our analysis supports the hypothesis that evolutionary expansion through tandem duplications of specific lipid gene families, particularly the stearoyl-acyl carrier protein desaturase (ShSAD) gene family, is the main driver of the abundance of ω-3-PUFAs in S. hispanica seeds. The insights gained from the genomic analysis of S. hispanica will help establish a molecular breeding target that can be leveraged through genome editing techniques to increase ω-3 content in oil crops.

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来源期刊
Plant Genome
Plant Genome PLANT SCIENCES-GENETICS & HEREDITY
CiteScore
6.00
自引率
4.80%
发文量
93
审稿时长
>12 weeks
期刊介绍: The Plant Genome publishes original research investigating all aspects of plant genomics. Technical breakthroughs reporting improvements in the efficiency and speed of acquiring and interpreting plant genomics data are welcome. The editorial board gives preference to novel reports that use innovative genomic applications that advance our understanding of plant biology that may have applications to crop improvement. The journal also publishes invited review articles and perspectives that offer insight and commentary on recent advances in genomics and their potential for agronomic improvement.
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