Physalis angulata L.对博莱霉素诱导的小鼠肺纤维化缓解相关基因表达的影响

Q2 Medicine
Journal of Experimental Pharmacology Pub Date : 2024-02-01 eCollection Date: 2024-01-01 DOI:10.2147/JEP.S439932
Ummul Khair Imaduddin, Afiat Berbudi, Enny Rohmawaty
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引用次数: 0

摘要

目的:为了探索 Physalis angulata L.(Ciplukan)提取物对博莱霉素诱导的小鼠模型肺纤维化缓解的潜在治疗效果,研究人员进行了一项综合研究。研究重点关注与纤维化进展相关的关键基因,包括Nox4、Mmp8、Klf4和FAS,并评估了服用Ciplukan提取物后它们的mRNA表达水平:方法:将博来霉素诱导的小鼠模型分为七组,研究西普鲁康提取物对纤维化相关基因表达的影响。小鼠皮下注射博莱霉素诱导肺纤维化,并给予不同剂量的西普鲁坎提取物治疗四周。通过半定量PCR分析Nox4、Klf4、Mmp8和FAS的肺纤维化mRNA表达:结果:服用西普鲁康提取物后,Nox4、Mmp8和Klf4的mRNA表达量分别显著下降(p值=0.000)、(p值=0.002)和(p值=0.007),这表明西普鲁康提取物具有潜在的抗纤维化作用。然而,FAS的表达保持不变(p值=0.127):结论:西普鲁康提取物对纤维化相关基因的表达,尤其是对Nox4、Mmp8和Klf4的表达有很好的影响。这项研究表明,该提取物具有干预纤维化进展的潜力,为治疗策略提供了潜在的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Physalis angulata L. Administration on Gene Expressions Related to Lung Fibrosis Resolution in Mice-Induced Bleomycin.

Purpose: To explore the potential therapeutic effects of Physalis angulata L. (Ciplukan) extract on lung fibrosis resolution in a Bleomycin-induced mouse model, researchers conducted a comprehensive study. The study focused on key genes associated with fibrosis progression, including Nox4, Mmp8, Klf4, and FAS, and assessed their mRNA expression levels following the administration of Ciplukan extract.

Methods: A Bleomycin-induced mice model was divided into seven groups to investigate the effects of ciplukan extract on fibrosis-related gene expressions. Mice were induced with subcutaneously injected Bleomycin to generate lung fibrosis and given different doses of the Ciplukan extract for four weeks. Lung fibrosis mRNA expression was analyzed by semi-quantitative PCR for Nox4, Klf4, Mmp8, and FAS.

Results: The administration of ciplukan extract resulted in a significant decrease in mRNA expression of Nox4 with p-value=0.000, Mmp8 with p-value =0.002, and Klf4 with p-value =0.007, indicating potential antifibrotic effects. However, FAS expression remained unchanged (p-value=0.127).

Conclusion: Ciplukan extract exhibited promising effects on fibrosis-related gene expressions, particularly Nox4, Mmp8, and Klf4. This study suggests that the extract has the potential to intervene in fibrosis progression, offering a potential avenue for therapeutic strategies.

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来源期刊
Journal of Experimental Pharmacology
Journal of Experimental Pharmacology Medicine-Pharmacology (medical)
CiteScore
7.40
自引率
0.00%
发文量
43
审稿时长
16 weeks
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