补充丁酸梭菌可缓解糖尿病小鼠的血管炎症

IF 6.8 2区 医学 Q1 ENDOCRINOLOGY & METABOLISM
Diabetes & Metabolism Journal Pub Date : 2024-05-01 Epub Date: 2024-02-02 DOI:10.4093/dmj.2023.0109
Tian Zhou, Shuo Qiu, Liang Zhang, Yangni Li, Jing Zhang, Donghua Shen, Ping Zhao, Lijun Yuan, Lianbi Zhao, Yunyou Duan, Changyang Xing
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引用次数: 0

摘要

背景:肠道微生物群与糖尿病的发生和发展密切相关,并影响糖尿病并发症的预后,而其潜在机制只有部分被了解。我们旨在探讨肠道微生物群与糖尿病小鼠血管炎症之间可能存在的联系:方法:本研究使用了 db/db 糖尿病小鼠和野生型(WT)小鼠。我们对 db/db 组和 WT 组小鼠的肠道微生物群进行了分析,并对其血管功能进行了检测。肠道微生物群通过 16s rRNA 测序进行分析。血管功能通过超声血流动力学和组织学染色进行检测。糖尿病小鼠每两天胃内灌胃一次丁酸梭菌(CB),连续灌胃两个月。荧光显微镜检测了活性氧(ROS)以及核因子红细胞衍生2相关因子2(Nrf2)和血红素加氧酶1(HO-1)的表达。定量聚合酶链反应检测了炎症细胞因子的 mRNA 表达:结果:与 WT 小鼠相比,db/db 小鼠肠道中的 CB 丰度明显降低,同时血管功能受损,动脉组织中的炎症被激活。同时,db/db 小鼠血管组织中的 ROS 也明显增加。口服 CB 恢复了保护性微生物群,并通过激活 Nrf2/HO-1 通路保护了 db/db 小鼠的血管功能:结论:这项研究发现了糖尿病患者肠道微生物群中 CB 丰度下降与血管炎症之间的潜在联系。结论:这项研究发现了肠道微生物群中 CB 丰度下降与糖尿病血管炎症之间的潜在联系,通过肠道移植治疗性输送 CB 可激活 Nrf2/HO-1 通路,从而缓解糖尿病的血管病变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Supplementation of Clostridium butyricum Alleviates Vascular Inflammation in Diabetic Mice.

Backgruound: Gut microbiota is closely related to the occurrence and development of diabetes and affects the prognosis of diabetic complications, and the underlying mechanisms are only partially understood. We aimed to explore the possible link between the gut microbiota and vascular inflammation of diabetic mice.

Methods: The db/db diabetic and wild-type (WT) mice were used in this study. We profiled gut microbiota and examined the and vascular function in both db/db group and WT group. Gut microbiota was analyzed by 16s rRNA sequencing. Vascular function was examined by ultrasonographic hemodynamics and histological staining. Clostridium butyricum (CB) was orally administered to diabetic mice by intragastric gavage every 2 days for 2 consecutive months. Reactive oxygen species (ROS) and expression of nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by fluorescence microscopy. The mRNA expression of inflammatory cytokines was tested by quantitative polymerase chain reaction.

Results: Compared with WT mice, CB abundance was significantly decreased in the gut of db/db mice, together with compromised vascular function and activated inflammation in the arterial tissue. Meanwhile, ROS in the vascular tissue of db/db mice was also significantly increased. Oral administration of CB restored the protective microbiota, and protected the vascular function in the db/db mice via activating the Nrf2/HO-1 pathway.

Conclusion: This study identified the potential link between decreased CB abundance in gut microbiota and vascular inflammation in diabetes. Therapeutic delivery of CB by gut transplantation alleviates the vascular lesions of diabetes mellitus by activating the Nrf2/HO-1 pathway.

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来源期刊
Diabetes & Metabolism Journal
Diabetes & Metabolism Journal Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
10.40
自引率
6.80%
发文量
92
审稿时长
52 weeks
期刊介绍: The aims of the Diabetes & Metabolism Journal are to contribute to the cure of and education about diabetes mellitus, and the advancement of diabetology through the sharing of scientific information on the latest developments in diabetology among members of the Korean Diabetes Association and other international societies. The Journal publishes articles on basic and clinical studies, focusing on areas such as metabolism, epidemiology, pathogenesis, complications, and treatments relevant to diabetes mellitus. It also publishes articles covering obesity and cardiovascular disease. Articles on translational research and timely issues including ubiquitous care or new technology in the management of diabetes and metabolic disorders are welcome. In addition, genome research, meta-analysis, and randomized controlled studies are welcome for publication. The editorial board invites articles from international research or clinical study groups. Publication is determined by the editors and peer reviewers, who are experts in their specific fields of diabetology.
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