茄子（Solanum melongena L.）结果习性基因座（Bh1）的遗传分析和分子图谱绘制

IF 1.5 4区农林科学 Q2 AGRONOMY

Plant Breeding Pub Date : 2024-01-30 DOI:10.1111/pbr.13161

Sulochana K. H., Partha Saha, Bhoopal Singh Tomar, Tusar Kanti Behera, Chandrika Ghoshal, Veerendra Kumar Verma, Aakriti Verma, Gopala Krishnan S, Namita Das Saha, Soham Ray, Sanjay Kumar Singh, Maganti Sheshu Madhav

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引用次数: 0

摘要

茄子有两种结果习性，即丛生和单生。丛生的果实体积小，但对产量和生产率有贡献。在这项调查中，我们研究了结实习性的遗传学，并确定了与这一性状相关的 SSR 标记。将性状截然不同的 Pusa Safed Baingan 1 号（丛生型）和 Pusa Hara Baingan 1 号（单生型）杂交，产生了 F1（20 株）、F2（215 株）、B1（73 株）和 B2（64 株）。性状的遗传分析采用卡方检验（Chi-square test）。F2 中植株的分离结果表明，隐性外显率为 9:3:4（单生：混合：丛生），表明有两个基因参与控制结实习性。亲本多态的 6 个 SSR 标记被用于 F2 植株的基因分型，并与结果习性（Bh1）基因座以 1:2:1 的比例共分离。绘制的连接图覆盖了 63.86 cm 的距离，其中 SSR 标记 emf11A03 的距离最近，为 4.37 cm，emk03O04 的距离为 12.5 cm，emf21O06 的距离为 14.5 cm。Bh1基因侧翼的SSR标记emb01J19和emf11A03的物理位置分别位于等位组Sme2.5_05721.1的4.77 mb位置和等位组Sme2.5_00994.1的5.18 Mb位置。本研究的发现将有助于通过标记辅助育种提高茄子产量。

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Genetic analysis and molecular mapping of fruit-bearing habit locus (Bh1) in eggplant (Solanum melongena L.)

In eggplant, two kinds of fruit-bearing habits can be observed, that is, cluster and solitary. The fruits grown in cluster are small in size but contribute towards yield and productivity. In this investigation, we have studied the genetics of fruit-bearing habit and identified the SSR markers linked to this trait. Pusa Safed Baingan 1 (cluster bearer) and Pusa Hara Baingan 1 (solitary bearer) having contrasting traits were crossed to generate F₁ (20 plants), F₂ (215 plants), B₁ (73 plants) and B₂ (64 plants). Genetic analysis of the trait was carried out using Chi-square test. The segregation of plants in F₂ suggested recessive epistasis with ratio of 9:3:4 (solitary: mixed: cluster) indicating involvement of two genes for controlling the fruit-bearing habit. The parental polymorphic 6 SSR markers were used for genotyping F₂ plants and co-segregated with the fruit-bearing habit (Bh¹) locus in 1:2:1. A linkage map covering 63.86 cm distance was developed, the SSR marker emf11A03 was closest with a distance of 4.37 cm, emk03O04 at 12.5 cm distance, and emf21O06 at 14.5 cm. The physical positions of the SSR markers emb01J19 and emf11A03 flanking Bh¹ gene were located at 4.77 mb position of contig Sme2.5_05721.1 and 5.18 Mb position of contig Sme2.5_00994.1, respectively. The findings in the present study will be helpful in marker-assisted breeding to enhance yield in eggplant.

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来源期刊

Plant Breeding 农林科学-农艺学

CiteScore

4.40

自引率

5.00%

发文量

审稿时长

3.0 months

期刊介绍： PLANT BREEDING publishes full-length original manuscripts and review articles on all aspects of plant improvement, breeding methodologies, and genetics to include qualitative and quantitative inheritance and genomics of major crop species. PLANT BREEDING provides readers with cutting-edge information on use of molecular techniques and genomics as they relate to improving gain from selection. Since its subject matter embraces all aspects of crop improvement, its content is sought after by both industry and academia. Fields of interest: Genetics of cultivated plants as well as research in practical plant breeding.