触觉刺激促进脑缺血大鼠运动功能的恢复

Neuro endocrinology letters Pub Date : 2024-01-31
Changkai Ke, Guangchen Yang, Heng Wang, Jiayi Hu, Chen Li, Chuan Huang, Chunxiao Wan
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引用次数: 0

摘要

目的:触觉刺激(TS)可促进缺氧缺血性脑损伤大鼠的神经发生和运动功能恢复,但其潜在机制尚不清楚。本研究旨在评估触觉刺激对脑缺血后大鼠神经功能的影响,并探讨其潜在机制:方法:将成年 SD 大鼠随机分为假手术(SHAM)组、大脑中动脉闭塞加触觉刺激(TS-MCAO)组和大脑中动脉闭塞加静坐干预(SED-MCAO)组。MCAO 24 小时后,TS-MCAO 组大鼠接受 20 分钟/天、5 天/周的 TS 刺激,持续 4 周。对大鼠的脑血流量(CBF)、体重变化、行为评分、梗死体积、皮质脊髓束完整性和神经化学变化进行了测量,并进行了Golgi-Cox染色、透射电子显微镜和Western印迹分析:结果:与 SED-MCAO 组相比,TS-MCAO 组的 CBF 恢复有所改善。干预 28 天后,TS-MCAO 组的体重和行为评分发生了显著变化。干预14天和28天后,梗死体积明显缩小,分数各向异性比率增加,表观扩散系数比率下降,乙酰天冬氨酸(NAA)/肌酸(Cr)和谷氨酸(Glu)/Cr比率增加。干预28天后,梗死周围皮层树突的复杂性和密度、突触数量以及突触可塑性相关蛋白的表达均有所增加:结论:TS能改善脑缺血大鼠的运动能力,而这种改善与突触可塑性相关。这一发现将有助于为脑卒中患者提供康复计划。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tactile stimulation promotes the recovery of motor function in rats with cerebral ischaemia.

Objectives: Tactile stimulation (TS) can promote neurogenesis and motor function recovery in rats with hypoxic-ischaemic brain injury, but the underlying mechanism is not clear. This study aimed to assess the effects of TS on neurological function in rats after cerebral ischaemia and explore the underlying mechanism.

Methods: Adult SD rats were randomly divided into a sham operation (SHAM) group, middle cerebral artery occlusion with tactile stimulation (TS-MCAO) group and middle cerebral artery occlusion with sedentary intervention (SED-MCAO) group. Twenty-four hours after MCAO, rats in the TS-MCAO group received TS for 20 min/d 5 d/w for 4 weeks. Cerebral blood flow (CBF), changes in body weight, behavioural scores, the infarct volume, corticospinal tract integrity, and neurochemical changes were measured, and Golgi-Cox staining, transmission electron microscopy and Western blotting were performed.

Results: CBF recovery was improved in the TS-MCAO group compared with the SED-MCAO group. Body weight and behavioural scores in the TS-MCAO group significantly changed after 28 days of intervention. After 14 and 28 days of intervention, the infarct volume decreased significantly, the ratios of fractional anisotropy increased and the ratios of apparent diffusion coefficient decreased, the ratios of Nacetylaspartate (NAA)/creatine (Cr) and glutamate (Glu)/ Cr increased. After 28 days of intervention, the complexity and density of dendrites, the number of synapses and the expression of synaptic plasticity-related proteins increased in the peri-infarct cortex.

Conclusion: TS can improve motor performance in rats with cerebral ischaemia and the improvement is correlated with synaptic plasticity. This finding would be helpful to provide a rehabilitation program for patients following stroke.

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