木聚糖酶在沸石咪唑酸框架-67 (ZIF-67) 和掺锰 ZIF-67 (Mn/ZIF-67) 中的固定化：比较研究

IF 2.8 3区化学 Q2 CHEMISTRY, APPLIED

Topics in Catalysis Pub Date : 2024-01-31 DOI:10.1007/s11244-023-01898-1

Büşra Bakar, Gamze Dik, Ahmet Ulu, Burhan Ateş

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引用次数: 0

摘要

人们发现，金属有机框架（MOFs）具有固定酶的理想特性，包括高表面积、巨大的内部孔隙和易于改变的孔径。本文首次采用原位法将木聚糖酶（Xyl）固定在两种不同的载体支撑物上，即沸石咪唑框架-67（ZIF-67）和掺锰的ZIF-67（Mn/ZIF-67）。通过傅立叶变换红外光谱（FT-IR）、X射线衍射（XRD）、扫描电镜（SEM）和EDAX技术对含有和不含Xyl的MOFs进行了物理化学表征。对 Xyl@ZIF-67 和 Xyl@Mn/ZIF-67 的最适温度、最适 pH 值、动力学参数、热稳定性、重复利用率以及果汁澄清度进行了评估，并与游离 Xyl 进行了比较。Xyl@ZIF-67 的最佳温度值为 50 °C，游离 Xyl 和 Xyl@Mn/ZIF-67 的最佳温度值为 70 °C。游离 Xyl、Xyl@ZIF-67 和 Xyl@Mn/ZIF-67 的最佳 pH 值分别为 6.0、8.0 和 7.0。游离 Xyl、Xyl@ZIF-67 和 Xyl@Mn/ZIF-67 的 Km 值分别为 3.139、5.430 和 0.799 mg/mL，Vmax 值分别为 0.167、0.226 和 0.062 µmol/min/mL。结果显示，与游离的 Xyl 相比，Xyl@ZIF-67 和 Xyl@Mn/ZIF-67 表现出更强的耐热性。在 70 °C 下孵育 120 分钟后，游离 Xyl 的活性保持在 28.7%，而 Xyl@ZIF-67 和 Xyl@Mn/ZIF-67 的活性分别保持在 85.7% 和 40.0%。此外，经过八个循环后，Xyl@ZIF-67 和 Xyl@Mn/ZIF-67 的活性保持在初始活性的 70% 以上。此外，使用 Xyl@ZIF-67 和 Xyl@Mn/ZIF-67 作为生物催化剂时，苹果汁的透光率分别从 65.61% 提高到 94.73%，从 77.80% 提高到 84.13%。总之，这些研究结果表明，所建议的Xyl@ZIF-67和Xyl@Mn/ZIF-67作为一种高效的异构生物催化剂在果汁澄清方面具有很大的潜力。图文摘要

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Immobilization of Xylanase into Zeolitic Imidazolate Framework-67 (ZIF-67) and Manganese-Doped ZIF-67 (Mn/ZIF-67): A Comparison Study

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Immobilization of Xylanase into Zeolitic Imidazolate Framework-67 (ZIF-67) and Manganese-Doped ZIF-67 (Mn/ZIF-67): A Comparison Study

It has been discovered that metal–organic frameworks (MOFs) have desirable qualities for the immobilization of enzymes, including a high surface area, significant interior pore volumes, and easily changeable pore size. Herein, the xylanase (Xyl) enzyme was immobilized for the first time to two different carrier supports, zeolitic imidazolate framework-67 (ZIF-67) and manganese-doped ZIF-67 (Mn/ZIF-67) by in situ method. The physicochemical characterizations of MOFs with and without Xyl were performed by FT-IR, XRD, SEM, and EDAX techniques. Xyl@ZIF-67 and Xyl@Mn/ZIF-67 were evaluated in terms of optimum temperature, optimum pH, kinetic parameters, thermal stability, reusability as well as juice clarification and compared with free Xyl. Optimum temperature values were 50 °C for Xyl@ZIF-67 and 70 °C for free Xyl and Xyl@Mn/ZIF-67. Optimum pH values for free Xyl, Xyl@ZIF-67, and Xyl@Mn/ZIF-67 were recorded as 6.0, 8.0, and 7.0, respectively. K_m values for free Xyl, Xyl@ZIF-67, and Xyl@Mn/ZIF-67 were calculated as 3.139, 5.430, and 0.799 mg/mL, respectively, while V_max values were calculated as 0.167, 0.226, and 0.062 µmol/min/mL, respectively. The results revealed that in comparison to the free Xyl, Xyl@ZIF-67, and Xyl@Mn/ZIF-67 exhibited more thermal resistance. After incubation at 70 °C for 120 min, the free Xyl remained at 28.7% of the activity, while the Xyl@ZIF-67 and Xyl@Mn/ZIF-67 remained at 85.7% and 40.0%, respectively. Moreover, after eight cycles, the Xyl@ZIF-67 and Xyl@Mn/ZIF-67 retained more than 70% of their initial activity. Further, the transmittance of apple juice was increased from 65.61 to 94.73% and from 77.80 to 84.13%, respectively, when Xyl@ZIF-67 and Xyl@Mn/ZIF-67 were used as biocatalysts. Overall, these findings indicated that the suggested Xyl@ZIF-67 and Xyl@Mn/ZIF-67 have a high potential for juice clarification as an efficient heterogeneous biocatalyst.

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来源期刊

Topics in Catalysis 化学-物理化学

CiteScore

5.70

自引率

5.60%

发文量

197

审稿时长

2 months

期刊介绍： Topics in Catalysis publishes topical collections in all fields of catalysis which are composed only of invited articles from leading authors. The journal documents today’s emerging and critical trends in all branches of catalysis. Each themed issue is organized by renowned Guest Editors in collaboration with the Editors-in-Chief. Proposals for new topics are welcome and should be submitted directly to the Editors-in-Chief. The publication of individual uninvited original research articles can be sent to our sister journal Catalysis Letters. This journal aims for rapid publication of high-impact original research articles in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis.