{"title":"表皮葡萄球菌菌株可抑制特应性皮炎皮肤中的金黄色葡萄球菌被角质细胞吸收","authors":"Tomofumi Numata, Kazumasa Iwamoto, Kyouka Matsunae, Ryu Miyake, Masataka Suehiro, Nozomi Yanagida, Takanobu Kan, Shunsuke Takahagi, Michihiro Hide, Akio Tanaka","doi":"10.1016/j.jdermsci.2024.01.006","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Various bacterial species form a microbiome in the skin. In the past, dead <em>Staphylococcus aureus</em> derived from atopic dermatitis (AD) are taken up by keratinocytes; however, whether live <em>S. aureus</em> can be taken up by keratinocytes is unknown.</p></div><div><h3>Objective</h3><p>This study aimed to examine whether live AD strains of <em>S. aureus</em> internalize into the keratinocytes and how the internalization changes under conditions in which other bacterial species including <em>S. epidermidis</em> are present.</p></div><div><h3>Methods</h3><p>HaCaT cells were cultured with live <em>S. aureus</em> and <em>S. epidermidis</em> (live or heat-treated) or their culture supernatants. After coculture, the change in the amount of <em>S. aureus</em> in the cytoplasm of HaCaT cells was analyzed using, a high-throughput imaging system, Opera Phenix™.</p></div><div><h3>Results</h3><p>Live <em>S. aureus</em> were taken up in the cytoplasm of HaCaT cells. Coculturing live <em>S. aureus</em> with live <em>S. epidermidis</em> or the culture supernatants decreased the abundance of <em>S. aureus</em> in the cytoplasm. The heat-treated culture supernatants of live <em>S. epidermidis</em> or culture supernatants of other <em>S.</em> strains did not decrease the abundance of <em>S. aureus</em> in the cytoplasm.</p></div><div><h3>Conclusion</h3><p>Live <em>S. aureus</em> was internalized into the cytoplasm of HaCaT cells as does heat-treated <em>S. aureus</em>. In addition, the heat-sensitive substances secreted by coculture with <em>S. epidermidis</em> and keratinocytes inhibited the uptake of <em>S. aureus</em> by keratinocytes.</p></div>","PeriodicalId":94076,"journal":{"name":"Journal of dermatological science","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Staphylococcus epidermidis strain inhibits the uptake of Staphylococcus aureus derived from atopic dermatitis skin into the keratinocytes\",\"authors\":\"Tomofumi Numata, Kazumasa Iwamoto, Kyouka Matsunae, Ryu Miyake, Masataka Suehiro, Nozomi Yanagida, Takanobu Kan, Shunsuke Takahagi, Michihiro Hide, Akio Tanaka\",\"doi\":\"10.1016/j.jdermsci.2024.01.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Various bacterial species form a microbiome in the skin. In the past, dead <em>Staphylococcus aureus</em> derived from atopic dermatitis (AD) are taken up by keratinocytes; however, whether live <em>S. aureus</em> can be taken up by keratinocytes is unknown.</p></div><div><h3>Objective</h3><p>This study aimed to examine whether live AD strains of <em>S. aureus</em> internalize into the keratinocytes and how the internalization changes under conditions in which other bacterial species including <em>S. epidermidis</em> are present.</p></div><div><h3>Methods</h3><p>HaCaT cells were cultured with live <em>S. aureus</em> and <em>S. epidermidis</em> (live or heat-treated) or their culture supernatants. After coculture, the change in the amount of <em>S. aureus</em> in the cytoplasm of HaCaT cells was analyzed using, a high-throughput imaging system, Opera Phenix™.</p></div><div><h3>Results</h3><p>Live <em>S. aureus</em> were taken up in the cytoplasm of HaCaT cells. Coculturing live <em>S. aureus</em> with live <em>S. epidermidis</em> or the culture supernatants decreased the abundance of <em>S. aureus</em> in the cytoplasm. The heat-treated culture supernatants of live <em>S. epidermidis</em> or culture supernatants of other <em>S.</em> strains did not decrease the abundance of <em>S. aureus</em> in the cytoplasm.</p></div><div><h3>Conclusion</h3><p>Live <em>S. aureus</em> was internalized into the cytoplasm of HaCaT cells as does heat-treated <em>S. aureus</em>. In addition, the heat-sensitive substances secreted by coculture with <em>S. epidermidis</em> and keratinocytes inhibited the uptake of <em>S. aureus</em> by keratinocytes.</p></div>\",\"PeriodicalId\":94076,\"journal\":{\"name\":\"Journal of dermatological science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of dermatological science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0923181124000148\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of dermatological science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0923181124000148","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
背景各种细菌在皮肤中形成微生物群。过去，特应性皮炎（AD）中死亡的金黄色葡萄球菌可被角质形成细胞吸收，但活的金黄色葡萄球菌能否被角质形成细胞吸收尚不清楚。方法用活的金黄色葡萄球菌和表皮葡萄球菌（活的或热处理的）或它们的培养上清培养 HaCaT 细胞。共培养后，使用高通量成像系统 Opera PhenixTM 分析 HaCaT 细胞胞质中金黄色葡萄球菌数量的变化。将活的金黄色葡萄球菌与活的表皮葡萄球菌或培养上清液共培养可减少细胞质中金黄色葡萄球菌的数量。结论活的金黄色葡萄球菌和热处理的金黄色葡萄球菌一样，都能内化到 HaCaT 细胞的细胞质中。此外，表皮葡萄球菌与角质形成细胞共培养分泌的热敏物质抑制了角质形成细胞对金黄色葡萄球菌的吸收。
A Staphylococcus epidermidis strain inhibits the uptake of Staphylococcus aureus derived from atopic dermatitis skin into the keratinocytes
Background
Various bacterial species form a microbiome in the skin. In the past, dead Staphylococcus aureus derived from atopic dermatitis (AD) are taken up by keratinocytes; however, whether live S. aureus can be taken up by keratinocytes is unknown.
Objective
This study aimed to examine whether live AD strains of S. aureus internalize into the keratinocytes and how the internalization changes under conditions in which other bacterial species including S. epidermidis are present.
Methods
HaCaT cells were cultured with live S. aureus and S. epidermidis (live or heat-treated) or their culture supernatants. After coculture, the change in the amount of S. aureus in the cytoplasm of HaCaT cells was analyzed using, a high-throughput imaging system, Opera Phenix™.
Results
Live S. aureus were taken up in the cytoplasm of HaCaT cells. Coculturing live S. aureus with live S. epidermidis or the culture supernatants decreased the abundance of S. aureus in the cytoplasm. The heat-treated culture supernatants of live S. epidermidis or culture supernatants of other S. strains did not decrease the abundance of S. aureus in the cytoplasm.
Conclusion
Live S. aureus was internalized into the cytoplasm of HaCaT cells as does heat-treated S. aureus. In addition, the heat-sensitive substances secreted by coculture with S. epidermidis and keratinocytes inhibited the uptake of S. aureus by keratinocytes.
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