产前缺氧导致成年大鼠和老龄大鼠海马中缺氧诱导因子 HIF1α 的含量和转录活性增加

IF 0.5 4区医学 Q4 NEUROSCIENCES

Neurochemical Journal Pub Date : 2023-12-01 DOI:10.1134/s1819712423330012

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引用次数: 0

摘要

摘要- 我们使用 Western 印迹法研究了缺氧诱导因子 1（HIF1α）调节α亚基在成年大鼠和衰老大鼠海马中的表达水平。此外，我们还利用定量实时 PCR 技术评估了成年大鼠体内 HIF1 调控基因（hk1、ldha、mct4、pdk1）mRNA 的相对丰度。我们的研究结果表明，产前缺氧会诱导海马中 HIF1α 表达的持续增加。此外，我们还观察到所研究的 HIF1 调控基因的 mRNA 水平随之升高。这些结果表明，产前缺氧会对海马的新陈代谢产生持久的影响。因此，这些代谢紊乱可能会持续终生。

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Prenatal Hypoxia Causes an Increase in the Content and Transcriptional Activity of the Hypoxia-Inducible Factor HIF1α in the Hippocampus of Adult and Aging Rats

Abstract—

Using Western blotting, we conducted a study investigating the expression levels of the regulatory alpha subunit of hypoxia-inducible factor 1 (HIF1α) in the hippocampus of adult and aging rats, whose mothers were exposed to severe damaging hypoxia during days 14-16 of pregnancy. Furthermore, we assessed the relative abundance of mRNA for HIF1-regulated genes (hk1, ldha, mct4, pdk1) using quantitative real-time PCR in adult rats. Our findings indicate that prenatal hypoxia induces a sustained increase in HIF1α expression in the hippocampus. Additionally, we observed an accompanying elevation in the mRNA levels of the investigated HIF1-regulated genes. These results suggest that prenatal hypoxia exerts a lasting impact on hippocampal metabolism. Consequently, these metabolic disruptions may persist throughout life.

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来源期刊

Neurochemical Journal 医学-神经科学

自引率

20.00%

发文量

审稿时长

>12 weeks

期刊介绍： Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components. The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.