加强混合螺旋介电泳微通道中的细胞分离:数值分析和最佳操作条件。

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Mohammed Raihan Uddin, Xiaolin Chen
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引用次数: 0

摘要

从血细胞中可靠地分离循环肿瘤细胞对于早期癌症诊断和预后至关重要。许多传统的微流控平台都是利用颗粒之间的尺寸差进行分离,这使得它们无法分拣重叠尺寸的细胞。为了解决这一问题,本文提出了一种惯性-电泳混合微流控芯片,用于连续、单级分离肺癌细胞株 A549 细胞和大小重叠的白细胞。实验结果验证了嵌入平面交错电极的螺旋微通道的工作机制。在一系列流动条件和电场强度下进行了数值研究,以确定细胞混合物的分离效率和迁移特性。研究结果表明,所提出的微通道具有独特的能力,能在垂直和横向两个方向上以较低的外加电压实现高通量的细胞分离。在四氯化碳和白细胞之间实现了明显的横向分离距离,从而实现了细胞的高分辨率和有效分离。分离分辨率可通过调整外加电场的强度来控制。此外,研究结果表明,横向分离距离在一个称为临界电压的电压下达到最大,该电压随着流速的增加而增加。所提出的微通道和所开发的技术可为开发一种可调且坚固耐用的医疗设备提供有价值的见解,该设备可有效、高通量地从白细胞中分离癌细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions

Enhancing cell separation in a hybrid spiral dielectrophoretic microchannel: Numerical insights and optimal operating conditions

Reliable separation of circulating tumor cells from blood cells is crucial for early cancer diagnosis and prognosis. Many conventional microfluidic platforms take advantage of the size difference between particles for their separation, which renders them impractical for sorting overlapping-sized cells. To address this concern, a hybrid inertial-dielectrophoretic microfluidic chip is proposed in this work for continuous and single-stage separation of lung cancer cell line A549 cells from white blood cells of overlapping size. The working mechanism of the proposed spiral microchannel embedded with planar interdigitated electrodes is validated against the experimental results. A numerical investigation is carried out over a range of flow conditions and electric field intensity to determine the separation efficiency and migration characteristics of the cell mixture. The results demonstrate the unique capability of the proposed microchannel to achieve high-throughput separation of cells at low applied voltages in both vertical and lateral directions. A significant lateral separation distance between the CTCs and the WBCs has been achieved, which allows for high-resolution and effective separation of cells. The separation resolution can be controlled by adjusting the strength of the applied electric field. Furthermore, the results demonstrate that the lateral separation distance is maximum at a voltage termed the critical voltage, which increases with the increase in the flow rate. The proposed microchannel and the developed technique can provide valuable insight into the development of a tunable and robust medical device for effective and high-throughput separation of cancer cells from the WBCs.

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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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