贾巴尔布尔地区反刍动物中副结核分枝杆菌亚种的分子和血清学检测

IF 0.4 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Indian Journal of Animal Research Pub Date : 2024-01-15 DOI:10.18805/ijar.b-5149

Y. Verma, Y. Verma, M. Swamy, A. Nayak, A. Dubey, M. Jatav, Amishi, A. Khare

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引用次数: 0

摘要

背景：副结核病是一种由副结核分枝杆菌（MAP）引起的野生和家养反刍动物慢性传染病。本研究旨在对贾巴尔布尔地区的反刍动物进行 MAP 的分子和血清学检测。研究方法从该地区的山羊、水牛和牛中收集了 261 份粪便样本、124 份血液样本和 51 份组织样本，不分年龄、性别和品种。首先对粪便和组织样本进行 ZN 染色，然后使用间接 ELISA 法检测血清样本中的抗 MAP 抗体，并对 ZN 阳性的粪便和组织样本进行 IS900 PCR 分析。结果根据 ZN 染色，反刍动物粪便样本中的酸性厌氧芽孢杆菌脱落模式为 +1，其中大多数为 +1。在酶联免疫吸附试验（ELISA）中，33.87%的血清样本发现抗 MAP 抗体呈阳性，而在 IS900 PCR 中，22.50% ZN 阳性的粪便样本和 100% ZN 阳性的组织样本均呈阳性。因此，可以得出贾巴尔布尔地区反刍动物 MAP 感染中度流行的结论。

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Molecular and Serological Detection of Mycobacterium avium Subspecies Paratuberculosis in Ruminants of Jabalpur Region

Background: Paratuberculosis is a chronic infectious disease of wild and domestic ruminants caused by Mycobacterium avium subspecies paratuberculosis (MAP). The present study aimed to conduct the molecular and serological detection of MAP in ruminants of Jabalpur region. Methods: A total of 261 faecal, 124 blood and 51 tissue samples were collected from goats, buffaloes and cattle of the region irrespective of their age, sex and breed. ZN staining of faecal and tissue samples was performed as a preliminary screening test followed by anti-MAP antibodies detection in serum samples using indirect ELISA and IS900 PCR analysis of ZN positive faecal and tissue samples. Result: A pattern of acid-fast bacilli shedding in faecal samples of ruminants was obtained based on ZN staining with a majority of +1 shedders. In ELISA, 33.87% of serum samples were found positive for anti-MAP antibodies whereas, 22.50% ZN positive faecal samples and 100% ZN positive tissue samples were found positive in IS900 PCR. Therefore, a conclusion of moderately prevalent MAP infection in ruminants of the Jabalpur region can be drawn.

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来源期刊

Indian Journal of Animal Research AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

1.00

自引率

20.00%

发文量

332

审稿时长

6 months

期刊介绍： The IJAR, the flagship print journal of ARCC, it is a monthly journal published without any break since 1966. The overall aim of the journal is to promote the professional development of its readers, researchers and scientists around the world. Indian Journal of Animal Research is peer-reviewed journal and has gained recognition for its high standard in the academic world. It anatomy, nutrition, production, management, veterinary, fisheries, zoology etc. The objective of the journal is to provide a forum to the scientific community to publish their research findings and also to open new vistas for further research. The journal is being covered under international indexing and abstracting services.