马铃薯(Solanum tuberosum L.)细胞悬浮培养的耐热效应评估

IF 2.3 3区 农林科学 Q1 AGRONOMY
M. Harun-Or-Rashid, S. M. Shahinul Islam, M. A. Bari Miah, Sreeramanan Subramaniam
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引用次数: 0

摘要

本研究探讨了热胁迫预处理因子对马铃薯细胞悬浮培养的影响。通过使用胰蓝排除法测量细胞的生长和活力,评估了在 5-50 ℃ 下 20 分钟的胁迫预处理持续时间。数据是通过测量悬浮细胞的干重、热胁迫持续时间、生长情况以及各时间点的生物量含量收集的。经 35 至 50 ℃ 热胁迫预处理后,细胞活力受到 85% 至 25% 的负面影响。然而,在恢复期间暴露于 30 ℃ 和 35 ℃ 的悬浮细胞在 55 小时后开始恢复活力,分别为 75% 和 69%。在 40 ℃ 或高温下处理的悬浮细胞中,观察到细胞活力完全下降,无法恢复生长。在马铃薯细胞悬浮培养物中,40 °C或更高温度下加热 20 分钟不仅会立即影响细胞活力,还会导致细胞随后死亡。有效的活力测试表明,马铃薯植株在细胞悬浮培养物中具有天然的耐热性。本研究确定的细胞对热反应的基本参数将有助于通过建立体外细胞悬浮培养物来选择耐热马铃薯品种。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluation of the Thermo-tolerance Effect on Cell Suspension Culture in Potato (Solanum tuberosum L.)

Evaluation of the Thermo-tolerance Effect on Cell Suspension Culture in Potato (Solanum tuberosum L.)

In this study, the effect of heat stress pretreatment factors on cell suspension culture of potato was investigated. The duration of stress pretreatment at 5–50 °C for 20 min was evaluated by measuring cell growth and viability using the trypan blue exclusion method. Data were collected by measuring the dry weight of suspended cells, duration of heat stress, growth, and biomass content at the respective time points. Cell viability was negatively affected by 85 to 25% after pretreatment with heat stress between 35 and 50 °C. However, suspended cells exposed to 30 °C and 35 °C during the recovery period began to regain viability at 75% and 69%, respectively, after 55 h. In suspended cells treated at 40 °C or high temperatures, a complete decrease in cell viability was observed, resulting in no recovery of growth. In potato cell suspension cultures, heat treatments at 40 °C or above for 20 min not only have an immediate effect on cell viability, but also lead to subsequent cell death. Effective viability tests showed the natural heat tolerance of potato plants in cell suspension cultures. The basic parameters of cellular response to heat determined in this study will be helpful in selecting heat-tolerant potato varieties by establishing in vitro cell suspension cultures.

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来源期刊
Potato Research
Potato Research AGRONOMY-
CiteScore
5.50
自引率
6.90%
发文量
66
审稿时长
>12 weeks
期刊介绍: Potato Research, the journal of the European Association for Potato Research (EAPR), promotes the exchange of information on all aspects of this fast-evolving global industry. It offers the latest developments in innovative research to scientists active in potato research. The journal includes authoritative coverage of new scientific developments, publishing original research and review papers on such topics as: Molecular sciences; Breeding; Physiology; Pathology; Nematology; Virology; Agronomy; Engineering and Utilization.
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