鼠伤寒沙门氏菌的基因网络分析揭示了参与应对低水活性的关键基因的新见解。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Nafiseh Davati, Abozar Ghorbani, Elham Ashrafi-Dehkordi, Thomas P Karbanowicz
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引用次数: 0

摘要

背景:当食源性细菌鼠伤寒沙门氏菌(Salmonella enterica serovar Typhimurium)暴露于渗透压时,细胞的适应性会增加毒力的严重性和细胞的存活率:Typhimurium 的基因网络分析旨在深入了解在低水活度(aw)条件下细胞存活所涉及基因之间的各种相互作用:我们使用 Cytoscape 对 72 小时后受到 aw 胁迫的三种食物样本进行了基因网络分析,以确定伤寒杆菌的基因簇和中心基因:结果:所发现的伤寒杆菌中心基因属于下调基因,与食物样本中的翻译、转录和核糖体结构有关。rpsB和Tig被确定为最重要的枢纽基因。中心基因的富集分析还显示了翻译和细胞蛋白质代谢过程的重要性。此外,还发现了牛奶巧克力中与有机氮代谢相关的生物过程。根据基因聚类分析的 KEGG 通路结果,细胞对压力的反应与 RNA 聚合酶、核糖体和氧化磷酸化有关。编码 RNA 聚合酶活性的基因,包括 rpoA、rpoB 和 rpoZ,也在 KEGG 通路中被显著识别。确定的枢纽 DEGs 主题包括 EXPREG_00000850、EXPREG_00000b00、EXPREG_000008e0 和 EXPREG_00000850:根据基因网络分析的结果,所发现的中枢基因可能有助于沙门氏菌对食物成分的适应,是沙门氏菌耐受低水压的原因。在各种食物样本中,牛奶巧克力基质导致了更多的鼠伤寒沙门氏菌生存适应途径,因为有更多的中枢基因被下调,并且检测到了更多的基序。所发现的基因主题涉及碳水化合物代谢、碳水化合物转运、电子传递和氧传递。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Gene Networks Analysis of Salmonella Typhimurium Reveals New Insights on Key Genes Involved in Response to Low Water Activity.

Background: When Salmonella enterica serovar Typhimurium, a foodborne bacterium, is exposed to osmotic stress, cellular adaptations increase virulence severity and cellular survival.

Objectives: The aim of the gene network analysis of S. Typhimurium was to provide insights into the various interactions between the genes involved in cellular survival under low water activity (aw).

Materials and methods: We performed a gene network analysis to identify the gene clusters and hub genes of S. Typhimurium using Cytoscape in three food samples subjected to aw stress after 72 hours.

Results: The identified hub genes of S. Typhimurium belonged to down-regulated genes and were related to translation, transcription, and ribosome structure in the food samples. The rpsB and Tig were identified as the most important of the hub genes. Enrichment analysis of the hub genes also revealed the importance of translation and cellular protein metabolic processes. Moreover, the biological process associated with organonitrogen metabolism in milk chocolate was identified. According to the KEGG pathway results of gene cluster analysis, cellular responses to stress were associated with RNA polymerase, ribosome, and oxidative phosphorylation. Genes encoding RNA polymerase activity, including rpoA, rpoB, and rpoZ, were also significantly identified in the KEGG pathways. The identified motifs of hub DEGs included EXPREG_00000850, EXPREG_00000b00, EXPREG_000008e0, and EXPREG_00000850.

Conclusion: Based on the results of the gene network analysis, the identified hub genes may contribute to adaptation to food compositions and be responsible for the development of low water stress tolerance in Salmonella. Among the food samples, the milk chocolate matrix leads to more adaptation pathways for S. Typhimurium survival, as more hub genes were down-regulated and more motifs were detected. The identified motifs were involved in carbohydrate metabolism, carbohydrate transport, electron transfer, and oxygen transfer.

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来源期刊
Iranian Journal of Biotechnology
Iranian Journal of Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
2.60
自引率
7.70%
发文量
20
期刊介绍: Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.
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