Kalpani H. Wijesinghe, Christopher Hood, Daniell Mattern, Laurence A. Angel, Amala Dass
{"title":"大体积叔丁基硫醇连接金纳米粒子的离子迁移-串联质谱法。","authors":"Kalpani H. Wijesinghe, Christopher Hood, Daniell Mattern, Laurence A. Angel, Amala Dass","doi":"10.1002/jms.4998","DOIUrl":null,"url":null,"abstract":"<p>Gold nanoparticles (AuNPs) synthesized in the 1–3 nm range have a specific number of gold core atoms and outer protecting ligands. They have become one of the “hot topics” in recent decades because of their interesting physical and chemical properties. The characterization of their structures is usually achieved by crystal X-ray diffraction although the structures of some AuNPs remain unknown because they have not been successfully crystallized. An alternative method for studying the structure of AuNPs is electrospray ionization–ion mobility–tandem mass spectrometry (ESI-IM-MSMS). This research evaluated how effectively ESI-IM-MSMS using the commercially available Waters Synapt XS instrument yielded useful structural information from two AuNPs; Au<sub>23</sub>(S-<i>t</i>Bu)<sub>16</sub> and Au<sub>30</sub>(S-<i>t</i>Bu)<sub>18</sub>. The study used the maximum range of available collision energies along with ion mobility separation to measure the energy-dependence of the product ions and their drift times which is a measure of their spatial size. For Au<sub>23</sub>(S-<i>t</i>Bu)<sub>16</sub>, the dissociation gave the masses of the outer protecting monomeric [RS–Au–SR] and trimeric [SR–Au–SR–Au–SR–Au–SR] staples where R = <i>t</i>Bu, and complete dissociation of the outer layer Au and <i>t</i>Bu groups to reveal the Au<sub>15</sub>S<sub>8</sub> core. For Au<sub>30</sub>(S-<i>t</i>Bu)<sub>18</sub>, the dissociation products was primarily through the loss of the partial ligands S-<i>t</i>Bu and <i>t</i>Bu from the outer protecting layer and the loss of single Au<sub>4</sub>(S-<i>t</i>Bu)<sub>4</sub> unit. These results showed the that ESI-IM-MSMS analysis of the smaller Au<sub>23</sub>(S-<i>t</i>Bu)<sub>16</sub> gave information on all it major structural components whereas for Au<sub>30</sub>(S-<i>t</i>Bu)<sub>18</sub>, the overall structural information was limited to the ligands of the outer layer.</p>","PeriodicalId":16178,"journal":{"name":"Journal of Mass Spectrometry","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ion mobility–tandem mass spectrometry of bulky tert-butyl thiol ligated gold nanoparticles\",\"authors\":\"Kalpani H. Wijesinghe, Christopher Hood, Daniell Mattern, Laurence A. Angel, Amala Dass\",\"doi\":\"10.1002/jms.4998\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Gold nanoparticles (AuNPs) synthesized in the 1–3 nm range have a specific number of gold core atoms and outer protecting ligands. They have become one of the “hot topics” in recent decades because of their interesting physical and chemical properties. The characterization of their structures is usually achieved by crystal X-ray diffraction although the structures of some AuNPs remain unknown because they have not been successfully crystallized. An alternative method for studying the structure of AuNPs is electrospray ionization–ion mobility–tandem mass spectrometry (ESI-IM-MSMS). This research evaluated how effectively ESI-IM-MSMS using the commercially available Waters Synapt XS instrument yielded useful structural information from two AuNPs; Au<sub>23</sub>(S-<i>t</i>Bu)<sub>16</sub> and Au<sub>30</sub>(S-<i>t</i>Bu)<sub>18</sub>. The study used the maximum range of available collision energies along with ion mobility separation to measure the energy-dependence of the product ions and their drift times which is a measure of their spatial size. For Au<sub>23</sub>(S-<i>t</i>Bu)<sub>16</sub>, the dissociation gave the masses of the outer protecting monomeric [RS–Au–SR] and trimeric [SR–Au–SR–Au–SR–Au–SR] staples where R = <i>t</i>Bu, and complete dissociation of the outer layer Au and <i>t</i>Bu groups to reveal the Au<sub>15</sub>S<sub>8</sub> core. For Au<sub>30</sub>(S-<i>t</i>Bu)<sub>18</sub>, the dissociation products was primarily through the loss of the partial ligands S-<i>t</i>Bu and <i>t</i>Bu from the outer protecting layer and the loss of single Au<sub>4</sub>(S-<i>t</i>Bu)<sub>4</sub> unit. These results showed the that ESI-IM-MSMS analysis of the smaller Au<sub>23</sub>(S-<i>t</i>Bu)<sub>16</sub> gave information on all it major structural components whereas for Au<sub>30</sub>(S-<i>t</i>Bu)<sub>18</sub>, the overall structural information was limited to the ligands of the outer layer.</p>\",\"PeriodicalId\":16178,\"journal\":{\"name\":\"Journal of Mass Spectrometry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-01-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Mass Spectrometry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jms.4998\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Mass Spectrometry","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jms.4998","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Ion mobility–tandem mass spectrometry of bulky tert-butyl thiol ligated gold nanoparticles
Gold nanoparticles (AuNPs) synthesized in the 1–3 nm range have a specific number of gold core atoms and outer protecting ligands. They have become one of the “hot topics” in recent decades because of their interesting physical and chemical properties. The characterization of their structures is usually achieved by crystal X-ray diffraction although the structures of some AuNPs remain unknown because they have not been successfully crystallized. An alternative method for studying the structure of AuNPs is electrospray ionization–ion mobility–tandem mass spectrometry (ESI-IM-MSMS). This research evaluated how effectively ESI-IM-MSMS using the commercially available Waters Synapt XS instrument yielded useful structural information from two AuNPs; Au23(S-tBu)16 and Au30(S-tBu)18. The study used the maximum range of available collision energies along with ion mobility separation to measure the energy-dependence of the product ions and their drift times which is a measure of their spatial size. For Au23(S-tBu)16, the dissociation gave the masses of the outer protecting monomeric [RS–Au–SR] and trimeric [SR–Au–SR–Au–SR–Au–SR] staples where R = tBu, and complete dissociation of the outer layer Au and tBu groups to reveal the Au15S8 core. For Au30(S-tBu)18, the dissociation products was primarily through the loss of the partial ligands S-tBu and tBu from the outer protecting layer and the loss of single Au4(S-tBu)4 unit. These results showed the that ESI-IM-MSMS analysis of the smaller Au23(S-tBu)16 gave information on all it major structural components whereas for Au30(S-tBu)18, the overall structural information was limited to the ligands of the outer layer.
期刊介绍:
The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions.
The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.